Home LiteratureArticle Details

PMID: 2418824 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Selection of a rat glutamine synthetase cDNA clone.

Biochemical and biophysical research communications ·Vol. 134 ·No. 1 ·1986-01-14 ·Pages 146-51

Burns DM, Bhandari B, Short JM, Sanders PG, Wilson RH, Miller RE

Abstract

We have selected a glutamine synthetase clone (pGSRK-1) from a rat kidney cDNA library. A partial restriction map has been constructed for the 1.65 kilobase pair (kbp) glutamine synthetase cDNA. Northern hybridization analysis indicates that 1) GS-specific RNA increases many-fold during adipocyte differentiation and 2) dexamethasone increases and insulin decreases GS-specific RNA in 3T3-L1 adipocytes.

MeSH Terms

Adipose Tissue Animals Cell Differentiation/drug effects Cell Line Cloning, Molecular DNA/analysis DNA Restriction Enzymes/metabolism Dexamethasone/pharmacology Glutamate-Ammonia Ligase/genetics Insulin/pharmacology Kidney/enzymology Nucleic Acid Hybridization RNA/metabolism Rats

Chemicals

Insulin RNA Dexamethasone DNA DNA Restriction Enzymes Glutamate-Ammonia Ligase

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Burns D M

Bhandari B

Short J M

Sanders P G

Wilson R H

Miller R E

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1016/0006-291x(86)90539-5

Published

1986-01-14

Pages

146-51

Language

English

Region

United States

NLM ID

0372516

Subset

Grants

NIADDK NIH HHS · 5-F32-AM07209 · United States

NIADDK NIH HHS · AM 21145 · United States

External Links

PubMed source DOI: 10.1016/0006-291x(86)90539-5 View journal details

Analysis Services

Selection of a rat glutamine synthetase cDNA clone.

Contact

WeChat Official Account