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PMID: 19648925 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Drosophila MSL complex globally acetylates H4K16 on the male X chromosome for dosage compensation.

Nature structural & molecular biology ·Vol. 16 ·No. 8 ·2009-08-00 ·Pages 825-32

Gelbart ME, Larschan E, Peng S, Park PJ, Kuroda MI

Abstract

The Drosophila melanogaster male-specific lethal (MSL) complex binds the single male X chromosome to upregulate gene expression to equal that from the two female X chromosomes. However, it has been puzzling that approximately 25% of transcribed genes on the X chromosome do not stably recruit MSL complex. Here we find that almost all active genes on the X chromosome are associated with robust H4 Lys16 acetylation (H4K16ac), the histone modification catalyzed by the MSL complex. The distribution of H4K16ac is much broader than that of the MSL complex, and our results favor the idea that chromosome-wide H4K16ac reflects transient association of the MSL complex, occurring through spreading or chromosomal looping. Our results parallel those of localized Polycomb repressive complex and its more broadly distributed chromatin mark, trimethylated histone H3 Lys27 (H3K27me3), suggesting a common principle for the establishment of active and silenced chromatin domains.

MeSH Terms
Acetylation Animals Blotting, Western Cell Line Chromatin Immunoprecipitation Dosage Compensation, Genetic Drosophila Proteins/genetics,metabolism Drosophila melanogaster/cytology,genetics,metabolism Female Gene Expression Profiling Histone Acetyltransferases/genetics,metabolism Histones/metabolism Lysine/metabolism Male Mutation RNA Interference X Chromosome/genetics
Chemicals
Drosophila Proteins Histones Histone Acetyltransferases Lysine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gelbart Marnie E
Division of Genetics, Department of Medicine, Brigham & Women's Hospital, Boston, Massachusetts, USA.
Larschan Erica
Peng Shouyong
Park Peter J
Kuroda Mitzi I
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Article Info
Journal
Nature structural & molecular biology
Abbr.
Nat Struct Mol Biol
ISSN
1545-9985
DOI
10.1038/nsmb.1644
Published
2009-08-00
Epub
2009-00-02
Pages
825-32
Language
English
Region
United States
NLM ID
101186374
PMCID
PMC2722042
Subset
IM
Grants
NIGMS NIH HHS · R01 GM082798 · United States
NIGMS NIH HHS · R01 GM082798-02 · United States
NIGMS NIH HHS · GM67825 · United States
NIGMS NIH HHS · K25 GM067825 · United States
NIGMS NIH HHS · R37 GM045744 · United States
NIGMS NIH HHS · R01 GM045744 · United States
NIGMS NIH HHS · GM45744 · United States
Databases
GEO
GSE14884
Corrections
CommentIn
PMID: 19654616
External Links
PubMed source DOI: 10.1038/nsmb.1644 PMC Full Text View journal details
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