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PMID: 17760505 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

ISWI regulates higher-order chromatin structure and histone H1 assembly in vivo.

PLoS biology ·Vol. 5 ·No. 9 ·2007-09-00 ·Pages e232

Corona DF, Siriaco G, Armstrong JA, Snarskaya N, McClymont SA, Scott MP, Tamkun JW

Abstract

Imitation SWI (ISWI) and other ATP-dependent chromatin-remodeling factors play key roles in transcription and other processes by altering the structure and positioning of nucleosomes. Recent studies have also implicated ISWI in the regulation of higher-order chromatin structure, but its role in this process remains poorly understood. To clarify the role of ISWI in vivo, we examined defects in chromosome structure and gene expression resulting from the loss of Iswi function in Drosophila. Consistent with a broad role in transcriptional regulation, the expression of a large number of genes is altered in Iswi mutant larvae. The expression of a dominant-negative form of ISWI leads to dramatic alterations in higher-order chromatin structure, including the apparent decondensation of both mitotic and polytene chromosomes. The loss of ISWI function does not cause obvious defects in nucleosome assembly, but results in a significant reduction in the level of histone H1 associated with chromatin in vivo. These findings suggest that ISWI plays a global role in chromatin compaction in vivo by promoting the association of the linker histone H1 with chromatin.

MeSH Terms
Adenosine Triphosphatases/deficiency,physiology Animals Chromatin/metabolism,ultrastructure Chromatin Assembly and Disassembly Drosophila Gene Expression Regulation Histones/analysis,metabolism Larva Mutation Transcription Factors/deficiency,physiology Transcription, Genetic
Chemicals
Chromatin Histones ISWI protein Transcription Factors Adenosine Triphosphatases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Corona Davide F V
Department of Molecular, Cell, and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America.
Siriaco Giorgia
Armstrong Jennifer A
Snarskaya Natalia
McClymont Stephanie A
Scott Matthew P
Tamkun John W
References (58)
58 references, click to expand
  1. Mitotic chromosome structure and condensation.
    Curr Opin Cell Biol. 2006 Dec;18(6):632-8 PMID: 17046228
  2. Dosage compensation: the beginning and end of generalization.
    Nat Rev Genet. 2007 Jan;8(1):47-57 PMID: 17173057
  3. Higher-order structures of chromatin: the elusive 30 nm fiber.
    Cell. 2007 Feb 23;128(4):651-4 PMID: 17320503
  4. Chromatin modifications and their function.
    Cell. 2007 Feb 23;128(4):693-705 PMID: 17320507
  5. The role of chromatin during transcription.
    Cell. 2007 Feb 23;128(4):707-19 PMID: 17320508
  6. Beyond the sequence: cellular organization of genome function.
    Cell. 2007 Feb 23;128(4):787-800 PMID: 17320514
  7. Histone modifications and the chromatin scaffold for meiotic chromosome architecture.
    Cell Cycle. 2006 Sep;5(18):2064-71 PMID: 16969105
  8. The ISWI chromatin-remodeling protein is required for gene expression and the maintenance of higher order chromatin structure in vivo.
    Mol Cell. 2000 Feb;5(2):355-65 PMID: 10882076
  9. Activation of transcription through histone H4 acetylation by MOF, an acetyltransferase essential for dosage compensation in Drosophila.
    Mol Cell. 2000 Feb;5(2):367-75 PMID: 10882077
  10. Critical role for the histone H4 N terminus in nucleosome remodeling by ISWI.
    Mol Cell Biol. 2001 Feb;21(3):875-83 PMID: 11154274
  11. Rapid exchange of histone H1.1 on chromatin in living human cells.
    Nature. 2000 Dec 14;408(6814):873-6 PMID: 11130728
  12. Dynamic binding of histone H1 to chromatin in living cells.
    Nature. 2000 Dec 14;408(6814):877-81 PMID: 11130729
  13. Decreased expression of specific genes in yeast cells lacking histone H1.
    J Biol Chem. 2001 Apr 27;276(17):13587-92 PMID: 11278859
  14. Histone H1 diversity: bridging regulatory signals to linker histone function.
    Gene. 2001 Jun 13;271(1):1-12 PMID: 11410360
  15. Histone tails modulate nucleosome mobility and regulate ATP-dependent nucleosome sliding by NURF.
    Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14316-21 PMID: 11724935
  16. A critical epitope for substrate recognition by the nucleosome remodeling ATPase ISWI.
    Nucleic Acids Res. 2002 Feb 1;30(3):649-55 PMID: 11809876
  17. Modulation of ISWI function by site-specific histone acetylation.
    EMBO Rep. 2002 Mar;3(3):242-7 PMID: 11882543
  18. Molecular biology. Chromatin higher order folding--wrapping up transcription.
    Science. 2002 Sep 13;297(5588):1824-7 PMID: 12228709
  19. Phosphorylation and an ATP-dependent process increase the dynamic exchange of H1 in chromatin.
    J Cell Biol. 2002 Sep 30;158(7):1161-70 PMID: 12356861
  20. Biological functions of the ISWI chromatin remodeling complex NURF.
    Genes Dev. 2002 Dec 15;16(24):3186-98 PMID: 12502740
  21. The Drosophila HOAP protein is required for telomere capping.
    Nat Cell Biol. 2003 Jan;5(1):82-4 PMID: 12510197
  22. H1 linker histones are essential for mouse development and affect nucleosome spacing in vivo.
    Mol Cell Biol. 2003 Jul;23(13):4559-72 PMID: 12808097
  23. Acf1 confers unique activities to ACF/CHRAC and promotes the formation rather than disruption of chromatin in vivo.
    Genes Dev. 2004 Jan 15;18(2):170-83 PMID: 14752009
  24. Genetic and cytological analysis of Drosophila chromatin-remodeling factors.
    Methods Enzymol. 2004;377:70-85 PMID: 14979019
  25. Multiple roles for ISWI in transcription, chromosome organization and DNA replication.
    Biochim Biophys Acta. 2004 Mar 15;1677(1-3):113-9 PMID: 15020052
  26. What functions do linker histones provide?
    Mol Microbiol. 2004 Aug;53(3):771-5 PMID: 15255891
  27. In vivo requirement of the RNA polymerase II elongation factor elongin A for proper gene expression and development.
    Mol Cell Biol. 2004 Nov;24(22):9911-9 PMID: 15509793
  28. HMG-D, the Drosophila melanogaster homologue of HMG 1 protein, is associated with early embryonic chromatin in the absence of histone H1.
    EMBO J. 1994 Apr 15;13(8):1817-22 PMID: 8168480
  29. Looking at Drosophila mitotic chromosomes.
    Methods Cell Biol. 1994;44:371-91 PMID: 7707964
  30. Ectopic expression in Drosophila.
    Methods Cell Biol. 1994;44:635-54 PMID: 7707973
  31. Linker histones are not essential and affect chromatin condensation in vivo.
    Cell. 1995 Jul 14;82(1):47-56 PMID: 7606784
  32. Identifying a transcription factor interaction site on RNA polymerase II.
    Gene Expr. 1995;5(1):49-69 PMID: 7488860
  33. ISWI, a member of the SWI2/SNF2 ATPase family, encodes the 140 kDa subunit of the nucleosome remodeling factor.
    Cell. 1995 Dec 15;83(6):1021-6 PMID: 8521502
  34. Linker histone H1 regulates specific gene expression but not global transcription in vivo.
    Cell. 1996 Aug 9;86(3):475-83 PMID: 8756729
  35. The biochemical and phenotypic characterization of Hho1p, the putative linker histone H1 of Saccharomyces cerevisiae.
    J Biol Chem. 1998 Mar 27;273(13):7268-76 PMID: 9516420
  36. The histone tails of the nucleosome.
    Curr Opin Genet Dev. 1998 Apr;8(2):140-6 PMID: 9610403
  37. decapentaplegic and wingless are regulated by eyes absent and eyegone and interact to direct the pattern of retinal differentiation in the eye disc.
    Development. 1998 Sep;125(18):3741-51 PMID: 9716539
  38. Analysis of Drosophila chromatin structure in vivo.
    Methods Enzymol. 1999;304:462-96 PMID: 10372377
  39. ACF consists of two subunits, Acf1 and ISWI, that function cooperatively in the ATP-dependent catalysis of chromatin assembly.
    Genes Dev. 1999 Jun 15;13(12):1529-39 PMID: 10385622
  40. Polytene chromosomes: 70 years of genetic research.
    Int Rev Cytol. 2004;241:203-75 PMID: 15548421
  41. Distinct activities of CHD1 and ACF in ATP-dependent chromatin assembly.
    Nat Struct Mol Biol. 2005 Feb;12(2):160-6 PMID: 15643425
  42. The dynamics of histone H1 function in chromatin.
    Mol Cell. 2005 Mar 4;17(5):617-20 PMID: 15749012
  43. The Drosophila trithorax group protein Kismet facilitates an early step in transcriptional elongation by RNA Polymerase II.
    Development. 2005 Apr;132(7):1623-35 PMID: 15728673
  44. Nucleosome and chromatin fiber dynamics.
    Curr Opin Struct Biol. 2005 Apr;15(2):188-96 PMID: 15837178
  45. Gene expression analysis of the function of the male-specific lethal complex in Drosophila.
    Genetics. 2005 Apr;169(4):2061-74 PMID: 15716510
  46. Histone H1 is essential for mitotic chromosome architecture and segregation in Xenopus laevis egg extracts.
    J Cell Biol. 2005 Jun 20;169(6):859-69 PMID: 15967810
  47. Two distinct mechanisms of chromatin interaction by the Isw2 chromatin remodeling complex in vivo.
    Mol Cell Biol. 2005 Nov;25(21):9165-74 PMID: 16227570
  48. The Drosophila nucleosome remodeling factor NURF is required for Ecdysteroid signaling and metamorphosis.
    Genes Dev. 2005 Nov 1;19(21):2540-5 PMID: 16264191
  49. Chromatin remodeling in dosage compensation.
    Annu Rev Genet. 2005;39:615-51 PMID: 16285873
  50. ATP-dependent chromatin remodeling complexes in Drosophila.
    Chromosome Res. 2006;14(4):433-49 PMID: 16821138
  51. Regulation of ISW2 by concerted action of histone H4 tail and extranucleosomal DNA.
    Mol Cell Biol. 2006 Oct;26(20):7388-96 PMID: 17015471
  52. Histone H1 depletion in mammals alters global chromatin structure but causes specific changes in gene regulation.
    Cell. 2005 Dec 29;123(7):1199-212 PMID: 16377562
  53. Histone H4-K16 acetylation controls chromatin structure and protein interactions.
    Science. 2006 Feb 10;311(5762):844-7 PMID: 16469925
  54. Role of linker histone in chromatin structure and function: H1 stoichiometry and nucleosome repeat length.
    Chromosome Res. 2006;14(1):17-25 PMID: 16506093
  55. Regulation of higher-order chromatin structures by nucleosome-remodelling factors.
    Curr Opin Genet Dev. 2006 Apr;16(2):151-6 PMID: 16503135
  56. The long and the short of it: linker histone H1 is required for metaphase chromosome compaction.
    Cell Cycle. 2006 Mar;5(6):589-91 PMID: 16582611
  57. Determinants of histone H1 mobility and chromatin binding in living cells.
    Nat Struct Mol Biol. 2006 Apr;13(4):305-10 PMID: 16715048
  58. Chromatin remodelling: the industrial revolution of DNA around histones.
    Nat Rev Mol Cell Biol. 2006 Jun;7(6):437-47 PMID: 16723979
Article Info
Journal
PLoS biology
Abbr.
PLoS Biol
ISSN
1545-7885
DOI
10.1371/journal.pbio.0050232
Published
2007-09-00
Pages
e232
Language
English
Region
United States
NLM ID
101183755
PMCID
PMC1951781
Subset
IM
Grants
NIGMS NIH HHS · GM49883 · United States
NIGMS NIH HHS · T32GM08646 · United States
NIGMS NIH HHS · T32 GM008646 · United States
Howard Hughes Medical Institute · United States
NIGMS NIH HHS · R01 GM049883 · United States
External Links
PubMed source DOI: 10.1371/journal.pbio.0050232 PMC Full Text View journal details
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