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PMID: 19644448 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of a perinuclear positioning element in human subtelomeres that requires A-type lamins and CTCF.

The EMBO journal ·Vol. 28 ·No. 16 ·2009-08-19 ·Pages 2428-36

Ottaviani A, Schluth-Bolard C, Rival-Gervier S, Boussouar A, Rondier D, Foerster AM, Morere J, Bauwens S, Gazzo S, Callet-Bauchu E, Gilson E, Magdinier F

Abstract

The localization of genes within the nuclear space is of paramount importance for proper genome functions. However, very little is known on the cis-acting elements determining subnuclear positioning of chromosome segments. We show here that the D4Z4 human subtelomeric repeat localizes a telomere at the nuclear periphery. This perinuclear activity lies within an 80 bp sequence included within a region known to interact with CTCF and A-type Lamins. We further show that a reduced level of either CTCF or A-type Lamins suppresses the perinuclear activities of D4Z4 and that an array of multimerized D4Z4 sequence, which has lost its ability to bind CTCF and A-type Lamins, is not localized at the periphery. Overall, these findings reveal the existence of an 80 bp D4Z4 sequence that is sufficient to position an adjacent telomere to the nuclear periphery in a CTCF and A-type lamins-dependent manner. Strikingly, this sequence includes a 30 bp GA-rich motif, which binds CTCF and is present at several locations in the human genome.

MeSH Terms
Animals Base Sequence Biological Transport CCCTC-Binding Factor Carcinoma/genetics,metabolism Cell Line, Tumor Cell Nucleolus/chemistry,metabolism Down-Regulation Epithelial Cells/cytology,metabolism Female Humans Insulator Elements Lamin Type A/metabolism Locus Control Region Protein Binding Repressor Proteins/genetics,metabolism Telomere/chemistry,metabolism Uterine Cervical Neoplasms/genetics,metabolism
Chemicals
CCCTC-Binding Factor CTCF protein, human Lamin Type A Repressor Proteins
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Ottaviani Alexandre
Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France.
Schluth-Bolard Caroline
Rival-Gervier Sylvie
Boussouar Amina
Rondier Delphine
Foerster Andrea M
Morere Julia
Bauwens Serge
Gazzo Sophie
Callet-Bauchu Evelyne
Gilson Eric
Magdinier Frédérique
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
1460-2075
DOI
10.1038/emboj.2009.201
Published
2009-08-19
Epub
2009-00-30
Pages
2428-36
Language
English
Region
England
NLM ID
8208664
PMCID
PMC2735173
Subset
IM
External Links
PubMed source DOI: 10.1038/emboj.2009.201 PMC Full Text View journal details
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