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PMID: 19618250 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DEAR1, a transcriptional repressor of DREB protein that mediates plant defense and freezing stress responses in Arabidopsis.

Journal of plant research ·Vol. 122 ·No. 6 ·2009-11-00 ·Pages 633-43

Tsutsui T, Kato W, Asada Y, Sako K, Sato T, Sonoda Y, Kidokoro S, Yamaguchi-Shinozaki K, Tamaoki M, Arakawa K, Ichikawa T, Nakazawa M, Seki M, Shinozaki K, Matsui M, Ikeda A, Yamaguchi J

Abstract

Plants have evolved intricate mechanisms to respond and adapt to a wide variety of biotic and abiotic stresses in their environment. The Arabidopsis DEAR1 (DREB and EAR motif protein 1; At3g50260) gene encodes a protein containing significant homology to the DREB1/CBF (dehydration-responsive element binding protein 1/C-repeat binding factor) domain and the EAR (ethylene response factor-associated amphiphilic repression) motif. We show here that DEAR1 mRNA accumulates in response to both pathogen infection and cold treatment. Transgenic Arabidopsis overexpressing DEAR1 (DEAR1ox) showed a dwarf phenotype and lesion-like cell death, together with constitutive expression of PR genes and accumulation of salicylic acid. DEAR1ox also showed more limited P. syringae pathogen growth compared to wild-type, consistent with an activated defense phenotype. In addition, transient expression experiments revealed that the DEAR1 protein represses DRE/CRT (dehydration-responsive element/C-repeat)-dependent transcription, which is regulated by low temperature. Furthermore, the induction of DREB1/CBF family genes by cold treatment was suppressed in DEAR1ox, leading to a reduction in freezing tolerance. These results suggest that DEAR1 has an upstream regulatory role in mediating crosstalk between signaling pathways for biotic and abiotic stress responses.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics,metabolism,physiology Arabidopsis Proteins/genetics Cloning, Molecular Freezing Gene Expression Profiling Molecular Sequence Data Repressor Proteins/chemistry,physiology Sequence Homology, Amino Acid Stress, Physiological Transcription Factors/genetics Transcription, Genetic/physiology
Chemicals
Arabidopsis Proteins DREB1A protein, Arabidopsis Repressor Proteins Transcription Factors
Authors & Affiliations
17 authors, click to expand affiliations / ORCID
Tsutsui Tomokazu
Faculty of Advanced Life Science, Graduate School of Life Science, Hokkaido University, Kita-ku N10-W8, Sapporo, 060-0810, Japan. ttsutsui@sci.hokudai.ac.jp
Kato Wataru
Asada Yutaka
Sako Kaori
Sato Takeo
Sonoda Yutaka
Kidokoro Satoshi
Yamaguchi-Shinozaki Kazuko
Tamaoki Masanori
Arakawa Keita
Ichikawa Takanari
Nakazawa Miki
Seki Motoaki
Shinozaki Kazuo
Matsui Minami
Ikeda Akira
Yamaguchi Junji
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Article Info
Journal
Journal of plant research
Abbr.
J Plant Res
ISSN
1618-0860
DOI
10.1007/s10265-009-0252-6
Email
ttsutsui@sci.hokudai.ac.jp
Published
2009-11-00
Epub
2009-00-18
Pages
633-43
Language
English
Region
Japan
NLM ID
9887853
Subset
IM
External Links
PubMed source DOI: 10.1007/s10265-009-0252-6 View journal details
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