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PMID: 19542374 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

A MyD88-dependent early IL-17 production protects mice against airway infection with the obligate intracellular pathogen Chlamydia muridarum.

Journal of immunology (Baltimore, Md. : 1950) ·Vol. 183 ·No. 2 ·2009-07-15 ·Pages 1291-300

Zhang X, Gao L, Lei L, Zhong Y, Dube P, Berton MT, Arulanandam B, Zhang J, Zhong G

Abstract

We found that IL-17, a signature cytokine of Th17, was produced early in the innate immunity phase after an intranasal infection with the obligate intracellular pathogen Chlamydia muridarum. The airway IL-17, which peaked at 48 h after infection, was dependent on live chlamydial organism replication and MyD88-mediated signaling pathways. Treatment with antibiotics or knockout of the MyD88 gene, but not Toll/IL receptor domain-containing adapter-inducing IFN-beta, can block the early IL-17 production. Treatment of mice with an anti-IL-17-neutralizing mAb enhanced growth of chlamydial organisms in the lung, dissemination to other organs, and decreased mouse survival, whereas treatment with an isotype-matched control IgG had no effect. Although IL-17 did not directly affect chlamydial growth in cell culture, it enhanced the production of other inflammatory cytokines and chemokines by Chlamydia-infected cells and promoted neutrophil infiltration in mouse airways during chlamydial infection, which may contribute to the antichlamydial effect of IL-17. These observations suggest that an early IL-17 response as an innate immunity component plays an important role in initiating host defense against infection with intracellular bacterial pathogens in the airway.

MeSH Terms
Animals Chlamydia muridarum/growth & development Cytokines/biosynthesis Immunity, Innate Interleukin-17/biosynthesis,immunology Mice Myeloid Differentiation Factor 88/immunology,physiology Neutrophil Infiltration Respiratory Tract Infections/immunology Survival Rate Time Factors
Chemicals
Cytokines Interleukin-17 Myeloid Differentiation Factor 88
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Zhang Xiaoyun
Department of Microbiology and Immunology, University of Texas Health Science Center, San Antonio, TX 78229, USA.
Gao Lifen
Lei Lei
Zhong Youmin
Dube Peter
Berton Michael T
Arulanandam Bernard
Zhang Jinshun
Zhong Guangming
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Article Info
Journal
Journal of immunology (Baltimore, Md. : 1950)
Abbr.
J Immunol
ISSN
1550-6606
DOI
10.4049/jimmunol.0803075
Published
2009-07-15
Epub
2009-00-19
Pages
1291-300
Language
English
Region
United States
NLM ID
2985117R
PMCID
PMC2810512
Subset
IM
Grants
NIAID NIH HHS · R01 AI057450 · United States
NIAID NIH HHS · R01 AI057450-04 · United States
External Links
PubMed source DOI: 10.4049/jimmunol.0803075 PMC Full Text View journal details
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