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PMID: 19477632 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Review

Engineering of recombinant crystallization chaperones.

Current opinion in structural biology ·Vol. 19 ·No. 4 ·2009-08-00 ·Pages 449-57

Koide S

Abstract

The preparation of diffraction quality crystals remains the major bottleneck in macromolecular X-ray crystallography. A crystallization chaperone is an auxiliary protein, such as fragments of monoclonal antibodies, that binds to and increases the crystallization probability of a target molecule of interest. Such chaperones reduce conformational heterogeneity, mask counterproductive surfaces while extending surfaces predisposed to forming crystal contacts, and provide phasing information. Crystallization chaperones generated using recombinant technologies have emerged as superior alternatives that increase the throughput and eliminate inherent limitations associated with antibody production by animal immunization and the hybridoma technology.

MeSH Terms
Animals Antibodies/metabolism Crystallization Humans Molecular Chaperones/chemistry,genetics,metabolism Protein Engineering/methods Protein Structure, Secondary Recombinant Proteins/chemistry,genetics,metabolism
Chemicals
Antibodies Molecular Chaperones Recombinant Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Koide Shohei
Department of Biochemistry and Molecular Biology, The University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA. skoide@uchicago.edu
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Article Info
Journal
Current opinion in structural biology
Abbr.
Curr Opin Struct Biol
ISSN
1879-033X
DOI
10.1016/j.sbi.2009.04.008
Email
skoide@uchicago.edu
Published
2009-08-00
Epub
2009-00-26
Pages
449-57
Language
English
Region
England
NLM ID
9107784
PMCID
PMC2736338
Subset
IM
Grants
NIGMS NIH HHS · U54 GM074946 · United States
NIGMS NIH HHS · R01 GM072688-01 · United States
NIDA NIH HHS · R21 DA025725 · United States
NIGMS NIH HHS · U54 GM074946-010002 · United States
NIDA NIH HHS · R21-DA025725 · United States
NIGMS NIH HHS · R01 GM072688 · United States
NCI NIH HHS · R21-CA132700 · United States
NIDA NIH HHS · R21 DA025725-01 · United States
NIGMS NIH HHS · U54-GM74946 · United States
NCI NIH HHS · R21 CA132700 · United States
NIGMS NIH HHS · R01-GM72688 · United States
NCI NIH HHS · R21 CA132700-01 · United States
External Links
PubMed source DOI: 10.1016/j.sbi.2009.04.008 PMC Full Text View journal details
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