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PMID: 17870469 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Review

Yeast surface display for protein engineering and characterization.

Current opinion in structural biology ·Vol. 17 ·No. 4 ·2007-08-00 ·Pages 467-73

Gai SA, Wittrup KD

Abstract

Yeast surface display is being employed to engineer desirable properties into proteins for a broad variety of applications. Labeling with soluble ligands enables rapid and quantitative analysis of yeast-displayed libraries by flow cytometry, while cell-surface selections allow screening of libraries with insoluble or even as-yet-uncharacterized binding targets. In parallel, the utilization of yeast surface display for protein characterization, including in particular the mapping of functional epitopes mediating protein-protein interactions, represents a significant recent advance.

MeSH Terms
Animals Enzymes, Immobilized/chemistry,metabolism Humans Peptide Library Protein Conformation Protein Engineering/methods Protein Interaction Mapping Saccharomyces cerevisiae/genetics Solubility
Chemicals
Enzymes, Immobilized Peptide Library
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gai S Annie
Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room E19-563, Cambridge, MA 02139, USA.
Wittrup K Dane
References (65)
65 references, click to expand
  1. High-affinity single-domain binding proteins with a binary-code interface.
    Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6632-7 PMID: 17420456
  2. Fine epitope mapping of anti-epidermal growth factor receptor antibodies through random mutagenesis and yeast surface display.
    J Mol Biol. 2004 Sep 10;342(2):539-50 PMID: 15327953
  3. Yeast surface display for screening combinatorial polypeptide libraries.
    Nat Biotechnol. 1997 Jun;15(6):553-7 PMID: 9181578
  4. SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling.
    Electrophoresis. 1997 Dec;18(15):2714-23 PMID: 9504803
  5. Yeast polypeptide fusion surface display levels predict thermal stability and soluble secretion efficiency.
    J Mol Biol. 1999 Oct 8;292(5):949-56 PMID: 10512694
  6. B and T lymphocyte attenuator exhibits structural and expression polymorphisms and is highly Induced in anergic CD4+ T cells.
    J Immunol. 2005 Mar 15;174(6):3377-85 PMID: 15749870
  7. Secretion and surface display of green fluorescent protein using the yeast Saccharomyces cerevisiae.
    Biotechnol Prog. 2005 Mar-Apr;21(2):349-57 PMID: 15801770
  8. Development of a humanized monoclonal antibody with therapeutic potential against West Nile virus.
    Nat Med. 2005 May;11(5):522-30 PMID: 15852016
  9. A general method for greatly improving the affinity of antibodies by using combinatorial libraries.
    Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8466-71 PMID: 15939870
  10. Design criteria for engineering inorganic material-specific peptides.
    Langmuir. 2005 Jul 19;21(15):6929-33 PMID: 16008405
  11. Molecular evolution of antibody affinity for sensitive detection of botulinum neurotoxin type A.
    J Mol Biol. 2005 Aug 5;351(1):158-69 PMID: 16002090
  12. Comprehensive antibody epitope mapping of the nucleocapsid protein of severe acute respiratory syndrome (SARS) coronavirus: insight into the humoral immunity of SARS.
    Clin Chem. 2005 Aug;51(8):1382-96 PMID: 15976093
  13. High-affinity CD25-binding IL-2 mutants potently stimulate persistent T cell growth.
    Biochemistry. 2005 Aug 9;44(31):10696-701 PMID: 16060678
  14. Enhanced reactivity of Rhizopus oryzae lipase displayed on yeast cell surfaces in organic solvents: potential as a whole-cell biocatalyst in organic solvents.
    Appl Environ Microbiol. 2005 Aug;71(8):4335-8 PMID: 16085821
  15. Serological identification of breast cancer-related antigens from a Saccharomyces cerevisiae surface display library.
    Int J Cancer. 2005 Oct 20;117(1):104-13 PMID: 15880534
  16. The use of scFv-displaying yeast in mammalian cell surface selections.
    J Immunol Methods. 2005 Sep;304(1-2):30-42 PMID: 16099466
  17. Structural basis of West Nile virus neutralization by a therapeutic antibody.
    Nature. 2005 Sep 29;437(7059):764-9 PMID: 16193056
  18. Characterization of T cell receptors engineered for high affinity against toxic shock syndrome toxin-1.
    J Mol Biol. 2005 Oct 21;353(2):308-21 PMID: 16171815
  19. Yeast surface display of a noncovalent MHC class II heterodimer complexed with antigenic peptide.
    Biotechnol Bioeng. 2005 Nov 20;92(4):485-91 PMID: 16155952
  20. Directed evolution for the development of conformation-specific affinity reagents using yeast display.
    Protein Eng Des Sel. 2005 Nov;18(11):527-36 PMID: 16186140
  21. Creation of Rhizopus oryzae lipase having a unique oxyanion hole by combinatorial mutagenesis in the lid domain.
    Appl Microbiol Biotechnol. 2005 Oct;68(6):779-85 PMID: 15729555
  22. Protective and therapeutic capacity of human single-chain Fv-Fc fusion proteins against West Nile virus.
    J Virol. 2005 Dec;79(23):14606-13 PMID: 16282460
  23. An immobilized biotin ligase: surface display of Escherichia coli BirA on Saccharomyces cerevisiae.
    Biotechnol Prog. 2005 Nov-Dec;21(6):1627-31 PMID: 16321044
  24. Prospective study on the expression of cancer testis genes and antibody responses in 100 consecutive patients with primary breast cancer.
    Int J Cancer. 2006 Feb 1;118(3):696-703 PMID: 16094643
  25. Class II-restricted T cell receptor engineered in vitro for higher affinity retains peptide specificity and function.
    Proc Natl Acad Sci U S A. 2005 Dec 27;102(52):19033-8 PMID: 16365315
  26. Antibodies against West Nile Virus nonstructural protein NS1 prevent lethal infection through Fc gamma receptor-dependent and -independent mechanisms.
    J Virol. 2006 Feb;80(3):1340-51 PMID: 16415011
  27. Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast.
    Proteins. 2006 Mar 1;62(4):1026-35 PMID: 16355407
  28. Construction and application of a yeast surface-displayed human cDNA library to identify post-translational modification-dependent protein-protein interactions.
    Mol Cell Proteomics. 2006 Mar;5(3):533-40 PMID: 16321969
  29. Effective display of metallothionein tandem repeats on the bioadsorption of cadmium ion.
    Appl Microbiol Biotechnol. 2006 Apr;70(4):458-63 PMID: 16091929
  30. Directed evolution to probe protein allostery and integrin I domains of 200,000-fold higher affinity.
    Proc Natl Acad Sci U S A. 2006 Apr 11;103(15):5758-63 PMID: 16595626
  31. Limitations of yeast surface display in engineering proteins of high thermostability.
    Protein Eng Des Sel. 2006 May;19(5):211-7 PMID: 16537642
  32. Serological immune response to cancer testis antigens in patients with pancreatic cancer.
    Int J Cancer. 2006 Jul 1;119(1):117-25 PMID: 16432832
  33. Probing the interface between biomolecules and inorganic materials using yeast surface display and genetic engineering.
    Acta Biomater. 2005 Mar;1(2):145-54 PMID: 16701791
  34. Development of a novel strategy for engineering high-affinity proteins by yeast display.
    Protein Eng Des Sel. 2006 Jun;19(6):255-64 PMID: 16549400
  35. Improved mutants from directed evolution are biased to orthologous substitutions.
    Protein Eng Des Sel. 2006 Jun;19(6):245-53 PMID: 16740523
  36. Isolation of anti-CD22 Fv with high affinity by Fv display on human cells.
    Proc Natl Acad Sci U S A. 2006 Jun 20;103(25):9637-42 PMID: 16763048
  37. Construction and characterization of a pseudo-immune human antibody library using yeast surface display.
    Biochem Biophys Res Commun. 2006 Aug 4;346(3):896-903 PMID: 16777066
  38. Rolling adhesion of alphaL I domain mutants decorrelated from binding affinity.
    J Mol Biol. 2006 Jun 30;360(1):37-44 PMID: 16813834
  39. Directed evolution for improved secretion of cancer-testis antigen NY-ESO-1 from yeast.
    Protein Expr Purif. 2006 Aug;48(2):232-42 PMID: 16563796
  40. Surface display of organophosphorus hydrolase on Saccharomyces cerevisiae.
    Biotechnol Prog. 2006 Jul-Aug;22(4):939-43 PMID: 16889366
  41. Application of the arming system for the expression of the 380R antigen from red sea bream iridovirus (RSIV) on the surface of yeast cells: a first step for the development of an oral vaccine.
    Biotechnol Prog. 2006 Jul-Aug;22(4):949-53 PMID: 16889368
  42. Development of novel yeast cell surface display system for homo-oligomeric protein by coexpression of native and anchored subunits.
    Biotechnol Prog. 2006 Jul-Aug;22(4):994-7 PMID: 16889375
  43. A flow cytometric assay for screening improved heterologous protein secretion in yeast.
    Biotechnol Prog. 2006 Jul-Aug;22(4):1200-8 PMID: 16889399
  44. The surface display of haemolysin from Vibrio harveyi on yeast cells and their potential applications as live vaccine in marine fish.
    Vaccine. 2006 Aug 28;24(35-36):6046-52 PMID: 16846670
  45. Cross-presentation of HLA class I epitopes from influenza matrix protein produced in Saccharomyces cerevisiae.
    Vaccine. 2006 Sep 11;24(37-39):6272-81 PMID: 16860448
  46. Engineering and characterization of a stabilized alpha1/alpha2 module of the class I major histocompatibility complex product Ld.
    J Biol Chem. 2006 Sep 1;281(35):25734-44 PMID: 16815841
  47. Immobilization of UDP-galactose 4-epimerase from Escherichia coli on the yeast cell surface.
    Biosci Biotechnol Biochem. 2006 Sep;70(9):2303-6 PMID: 16960363
  48. Cell surface expression of bacterial esterase A by Saccharomyces cerevisiae and its enhancement by constitutive activation of the cellular unfolded protein response.
    Appl Environ Microbiol. 2006 Nov;72(11):7140-7 PMID: 16980424
  49. Antibody recognition and neutralization determinants on domains I and II of West Nile Virus envelope protein.
    J Virol. 2006 Dec;80(24):12149-59 PMID: 17035317
  50. Method for generation of in vivo biotinylated recombinant antibodies by yeast mating.
    J Immunol Methods. 2006 Dec 20;317(1-2):132-43 PMID: 17113097
  51. Fine and domain-level epitope mapping of botulinum neurotoxin type A neutralizing antibodies by yeast surface display.
    J Mol Biol. 2007 Jan 5;365(1):196-210 PMID: 17059824
  52. Molecular evolution of antibody cross-reactivity for two subtypes of type A botulinum neurotoxin.
    Nat Biotechnol. 2007 Jan;25(1):107-16 PMID: 17173035
  53. Mining a yeast library for brain endothelial cell-binding antibodies.
    Nat Methods. 2007 Feb;4(2):143-5 PMID: 17206151
  54. Evolution of an interloop disulfide bond in high-affinity antibody mimics based on fibronectin type III domain and selected by yeast surface display: molecular convergence with single-domain camelid and shark antibodies.
    J Mol Biol. 2007 May 11;368(4):1024-41 PMID: 17382960
  55. Improvement of a recombinant anti-monkey anti-CD3 diphtheria toxin based immunotoxin by yeast display affinity maturation of the scFv.
    Bioconjug Chem. 2007 May-Jun;18(3):947-55 PMID: 17352456
  56. Construction of a novel synergistic system for production and recovery of secreted recombinant proteins by the cell surface engineering.
    Appl Microbiol Biotechnol. 2007 Jun;75(4):821-8 PMID: 17345082
  57. Improved bread-baking process using Saccharomyces cerevisiae displayed with engineered cyclodextrin glucanotransferase.
    J Agric Food Chem. 2007 Jun 13;55(12):4735-40 PMID: 17488117
  58. Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity.
    Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):10701-5 PMID: 10984501
  59. Production of complex human glycoproteins in yeast.
    Science. 2003 Aug 29;301(5637):1244-6 PMID: 12947202
  60. Neutralization of staphylococcal enterotoxin B by soluble, high-affinity receptor antagonists.
    Nat Med. 2007 Jun;13(6):725-9 PMID: 17515896
  61. Metallopeptidase, neurolysin, as a novel molecular tool for analysis of properties of cancer-producing matrix metalloproteinases-2 and -9.
    Appl Microbiol Biotechnol. 2007 Jul;75(6):1285-91 PMID: 17404728
  62. Peptide tags for enhanced cellular and protein adhesion to single-crystalline sapphire.
    Biotechnol Bioeng. 2007 Aug 1;97(5):1009-20 PMID: 17238208
  63. A novel high-throughput screen reveals yeast genes that increase secretion of heterologous proteins.
    Appl Environ Microbiol. 2007 Feb;73(4):1189-98 PMID: 17189442
  64. Antigen selection from an HIV-1 immune antibody library displayed on yeast yields many novel antibodies compared to selection from the same library displayed on phage.
    Protein Eng Des Sel. 2007 Feb;20(2):81-90 PMID: 17242026
  65. Isolating and engineering human antibodies using yeast surface display.
    Nat Protoc. 2006;1(2):755-68 PMID: 17406305
Article Info
Journal
Current opinion in structural biology
Abbr.
Curr Opin Struct Biol
ISSN
0959-440X
DOI
10.1016/j.sbi.2007.08.012
Published
2007-08-00
Epub
2007-00-17
Pages
467-73
Language
English
Region
England
NLM ID
9107784
PMCID
PMC4038029
Subset
IM
Grants
NCI NIH HHS · CA101830 · United States
NIAID NIH HHS · R01 AI065824 · United States
NCI NIH HHS · R01 CA101830 · United States
NIAID NIH HHS · AI065824 · United States
NCI NIH HHS · R01 CA096504 · United States
External Links
PubMed source DOI: 10.1016/j.sbi.2007.08.012 PMC Full Text View journal details
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