Abstract
Electrophysiological, biochemical, and Ca2+ imaging studies of cultured mouse myotubes were used to investigate whether the neurotransmitter acetylcholine causes an increase in intracellular Ca2+ concentration ([Ca2+]i) through activation of a second messenger system. Bath applications of acetylcholine to myotubes (i) elicited a significant membrane current even in a Na(+)-free Ca2+ medium, when the current was carried mainly by calcium ions; (ii) caused a rapid and transient cytosolic accumulation of inositol 1,4,5-trisphosphate; (iii) evoked a conspicuous alpha-bungarotoxin-sensitive long-lasting [Ca2+]i enhancement even in the presence of Cd2+; and (iv) transiently increased [Ca2+]i when cells were equilibrated in a Ca(2+)-free atropine-containing medium. We propose that, in addition to opening ion channels, the nicotinic action of acetylcholine on the muscle cell membrane increases [Ca2+]i through activation of the inositol 1,4,5-trisphosphate second messenger system and mobilization of Ca2+ from intracellular stores.
MeSH Terms
Acetylcholine/pharmacology
Animals
Calcium/metabolism,pharmacology
Cations
Cell Line
Cell Membrane/drug effects,physiology
Cytosol/drug effects,metabolism
Evoked Potentials/drug effects
Fura-2
Inositol 1,4,5-Trisphosphate/metabolism
Inositol Phosphates/metabolism
Kinetics
Mice
Muscles/physiology
Phosphatidylinositols/metabolism
Potassium/pharmacology
Receptors, Cholinergic/drug effects,physiology
Ribonucleotides/metabolism
Second Messenger Systems/drug effects
Signal Transduction
Spectrometry, Fluorescence
Chemicals
Cations
Inositol Phosphates
Phosphatidylinositols
Receptors, Cholinergic
Ribonucleotides
Inositol 1,4,5-Trisphosphate
Acetylcholine
Potassium
Calcium
Fura-2
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Giovannelli A
Laboratorio di Biofisica, Centro della Ricerca Sperimentale Istituto Regina Elena, Rome, Italy.
Grassi F
Mattei E
Mileo A M
Eusebi F
Giovanelli A
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