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PMID: 1945848 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A Drosophila Adh gene can be activated in trans by an enhancer.

Nucleic acids research ·Vol. 19 ·No. 20 ·1991-10-25 ·Pages 5713-7

Rothberg I, Hotaling E, Sofer W

Abstract

The ability of a segment of the Drosophila Adh gene to produce ADH activity in larvae is dependent upon the presence of a 53 bp sequence (called NS1) located between 289 and 341 bp upstream of the larval transcription start site. This sequence behaves like an enhancer in that it can stimulate gene activity when it is placed at various distances from, or on either side of, an Adh gene. Like a typical enhancer, NS1 does not ordinarily function in trans. However, when an Adh gene lacking NS1 is placed on one plasmid, and a second gene carrying NS1 is placed on another, and the two plasmids are interlocked in a catenane, both genes are active. This finding supports the mechanism of loop-mediated enhancer action.

Related Genes
Adh
MeSH Terms
Alcohol Dehydrogenase/genetics,metabolism Animals Base Sequence Blotting, Southern Cloning, Molecular DNA Drosophila melanogaster/enzymology,genetics Enhancer Elements, Genetic Gene Expression Regulation, Enzymologic Molecular Sequence Data Plasmids Transcriptional Activation
Chemicals
DNA Alcohol Dehydrogenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rothberg I
Department of Chemistry, Rutgers, State University of New Jersey, Newark 07102.
Hotaling E
Sofer W
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28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.20.5713
Published
1991-10-25
Pages
5713-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328980
Subset
IM
Grants
NIGMS NIH HHS · GM28791 · United States
External Links
PubMed source DOI: 10.1093/nar/19.20.5713 PMC Full Text View journal details
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