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PMID: 1937764 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Murine macrophage activation by staphylococcal exotoxins.

Infection and immunity ·Vol. 59 ·No. 11 ·1991-11-00 ·Pages 4049-55

Fleming SD, Iandolo JJ, Chapes SK

Abstract

We investigated the ability of staphylococcal enterotoxins A and B, exfoliative toxins A and B, and toxic shock syndrome toxin 1 to activate macrophages. All of the toxins tested had the potential to stimulate tumoricidal activity in peritoneal macrophages from lipopolysaccharide-responsive C3HeB/FeJ mice. In contrast, none of the toxins activated cytotoxicity in lipopolysaccharide-unresponsive macrophages from C3H/HeJ mice. We also studied toxin stimulation of monokine secretion. Staphylococcal enterotoxin A, toxic shock syndrome toxin 1, and both exfoliative toxins triggered C3HeB/FeJ macrophages to secrete tumor necrosis factor alpha, but enterotoxin B induced only marginal amounts of tumor necrosis factor. All of the toxins used stimulated interleukin-6 production by macrophages from both strains of mice. Nitric oxide is produced in response to the exfoliative toxins only by the lipopolysaccharide-responsive macrophages. These results suggest that macrophages respond differently to several staphylococcal exotoxins.

MeSH Terms
Animals Bacterial Toxins/immunology Cytotoxicity, Immunologic Enterotoxins/immunology Exfoliatins/immunology Exotoxins/immunology Interferon-gamma/pharmacology Interleukin-6/metabolism Macrophage Activation Macrophages/immunology Mice Mice, Inbred C3H Nitric Oxide/metabolism Nitrites/metabolism Recombinant Proteins Staphylococcus aureus/immunology,pathogenicity Superantigens Time Factors Tumor Necrosis Factor-alpha/metabolism
Chemicals
Bacterial Toxins Enterotoxins Exfoliatins Exotoxins Interleukin-6 Nitrites Recombinant Proteins Superantigens Tumor Necrosis Factor-alpha enterotoxin F, Staphylococcal Nitric Oxide enterotoxin A, Staphylococcal enterotoxin B, staphylococcal Interferon-gamma
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fleming S D
Division of Biology, Kansas State University, Manhattan 66506.
Iandolo J J
Chapes S K
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.11.4049-4055.1991
Published
1991-11-00
Pages
4049-55
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258995
Subset
IM
Grants
NIAID NIH HHS · AI-17474 · United States
External Links
PubMed source DOI: 10.1128/iai.59.11.4049-4055.1991 PMC Full Text View journal details
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