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PMID: 3500130 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Synergistic induction of interleukin-1 by endotoxin and toxic shock syndrome toxin-1 using rat macrophages.

Infection and immunity ·Vol. 55 ·No. 12 ·1987-12-00 ·Pages 2865-9

Beezhold DH, Best GK, Bonventre PF, Thompson M

Abstract

We studied interleukin-1 (IL-1) secretion by rat peritoneal exudate macrophages stimulated with purified toxic shock syndrome toxin-1 (TSST-1). TSST-1 was observed to be a more potent inducer of IL-1 than was endotoxin. The induction of IL-1 secretion by TSST-1 was not blocked by polymyxin B but could be blocked by monoclonal antibodies directed against TSST-1. Synergistic induction of IL-1 was observed when the cells were stimulated with TSST-1 and endotoxin. The sequence of addition was found to be important for the synergistic response. Enhanced IL-1 production was observed only when macrophages were exposed to endotoxin before or simultaneously with TSST-1. Prior exposure of macrophages to TSST-1 had no enhancing effect on endotoxin-induced IL-1 secretion. We conclude that stimulation of the macrophage by endotoxin enhances the responsiveness of the cells to TSST-1 and may thereby play a role in the pathogenesis of toxic shock syndrome.

MeSH Terms
Animals Antibodies, Monoclonal Bacterial Toxins Biological Assay Drug Synergism Endotoxins/pharmacology Enterotoxins/immunology,pharmacology Interleukin-1/biosynthesis Lymphocyte Activation/drug effects Macrophages/physiology Rats Superantigens
Chemicals
Antibodies, Monoclonal Bacterial Toxins Endotoxins Enterotoxins Interleukin-1 Superantigens enterotoxin F, Staphylococcal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Beezhold D H
Department of Anatomy, Medical College of Georgia, Augusta 30912.
Best G K
Bonventre P F
Thompson M
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28 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.12.2865-2869.1987
Published
1987-12-00
Pages
2865-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259999
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.55.12.2865-2869.1987 PMC Full Text View journal details
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