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PMID: 1935904 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleocytoplasmic shuttling of the progesterone receptor.

The EMBO journal ·Vol. 10 ·No. 12 ·1991-12-00 ·Pages 3851-9

Guiochon-Mantel A, Lescop P, Christin-Maitre S, Loosfelt H, Perrot-Applanat M, Milgrom E

Abstract

The nuclear localization of the progesterone receptor is mediated by two signal sequences: one is constitutive and lies in the hinge region (between the DNA and steroid binding domains), the other is hormone dependent and is localized in the second zinc finger of the DNA binding domain. The use of various inhibitors of energy synthesis in cells expressing permanently or transiently the wild-type receptor or a receptor mutated within the nuclear localization signals, demonstrated that the nuclear residency of the receptor reflects a dynamic situation: the receptor diffusing into the cytoplasm and being constantly and actively transported back into the nucleus. The existence of this nucleo-cytoplasmic shuttle mechanism was confirmed by receptor transfer from one nucleus to the other in heterokaryons. Preliminary evidence was obtained, using oestrogen receptor, that this phenomenon may be of general significance for steroid receptors.

MeSH Terms
Animals Biological Transport, Active Cell Line Cell Nucleus/metabolism Cytoplasm/metabolism DNA/genetics Fluorescent Antibody Technique Humans L Cells Mice Microinjections Plasmids Protein Sorting Signals/metabolism Receptors, Estrogen/metabolism Receptors, Progesterone/biosynthesis,metabolism Transfection
Chemicals
Protein Sorting Signals Receptors, Estrogen Receptors, Progesterone DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Guiochon-Mantel A
Hormones et Reproduction INSERM U 135, Faculté de Médecine Le Kremlin Bicêtre, France.
Lescop P
Christin-Maitre S
Loosfelt H
Perrot-Applanat M
Milgrom E
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-12-00
Pages
3851-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC453122
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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