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PMID: 2100202 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Signal transduction by steroid hormones: nuclear localization is differentially regulated in estrogen and glucocorticoid receptors.

Cell regulation ·Vol. 1 ·No. 3 ·1990-02-00 ·Pages 291-9

Picard D, Kumar V, Chambon P, Yamamoto KR

Abstract

The glucocorticoid receptor accumulates in nuclei only in the presence of bound hormone, whereas the estrogen receptor has been reported to be constitutively nuclear. To investigate this distinction, we compared the nuclear localization domains of the two receptors and the capacity of their respective hormone-binding regions to regulate nuclear localization activity. As with the glucocorticoid receptor, we showed that the human estrogen receptor contained a nuclear localization signal between the DNA-binding and hormone-binding regions (amino acids 256-303); however, in contrast to the glucocorticoid receptor, the estrogen receptor lacked a second nuclear localization domain within the hormone-binding region. Moreover, the hormone-binding domain of the unliganded estrogen receptor failed to regulate nuclear localization signals, although it efficiently regulated other receptor functions. We conclude that the two receptors employ a common mechanism for signal transduction involving a novel "inactivation" function, but that they differ in their control of nuclear localization. Thus, despite the strong relatedness of the estrogen and glucocorticoid receptors in structure and activity, certain differences in their properties could have important functional implications.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Nucleus/metabolism Cells, Cultured Chlorocebus aethiops DNA-Binding Proteins/metabolism Glucocorticoids/physiology Models, Biological Molecular Sequence Data Receptors, Estrogen/metabolism Receptors, Glucocorticoid/metabolism Recombinant Fusion Proteins/metabolism Signal Transduction Steroids/physiology
Chemicals
DNA-Binding Proteins Glucocorticoids Receptors, Estrogen Receptors, Glucocorticoid Recombinant Fusion Proteins Steroids
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Picard D
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.
Kumar V
Chambon P
Yamamoto K R
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Article Info
Journal
Cell regulation
Abbr.
Cell Regul
ISSN
1044-2030
DOI
10.1091/mbc.1.3.291
Published
1990-02-00
Pages
291-9
Language
English
Region
United States
NLM ID
9005331
PMCID
PMC361473
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.1.3.291 PMC Full Text
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