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PMID: 19352404 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

Ubiquitin-like protein activation by E1 enzymes: the apex for downstream signalling pathways.

Nature reviews. Molecular cell biology ·Vol. 10 ·No. 5 ·2009-05-00 ·Pages 319-31

Schulman BA, Harper JW

Abstract

Attachment of ubiquitin or ubiquitin-like proteins (known as UBLs) to their targets through multienzyme cascades is a central mechanism to modulate protein functions. This process is initiated by a family of mechanistically and structurally related E1 (or activating) enzymes. These activate UBLs through carboxy-terminal adenylation and thiol transfer, and coordinate the use of UBLs in specific downstream pathways by charging cognate E2 (or conjugating) enzymes, which then interact with the downstream ubiquitylation machinery to coordinate the modification of the target. A broad understanding of how E1 enzymes activate UBLs and how they selectively coordinate UBLs with downstream function has come from enzymatic, structural and genetic studies.

MeSH Terms
Animals Humans Signal Transduction/physiology Ubiquitin/metabolism Ubiquitin-Activating Enzymes/metabolism Ubiquitination Ubiquitins/metabolism
Chemicals
Ubiquitin Ubiquitins Ubiquitin-Activating Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schulman Brenda A
Howard Hughes Medical Institute, Department of Structural Biology, St Jude Children's Research Hospital, Memphis, Tennessee 38105, USA. brenda.schulman@stjude.org
Harper J Wade
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Article Info
Journal
Nature reviews. Molecular cell biology
Abbr.
Nat Rev Mol Cell Biol
ISSN
1471-0080
DOI
10.1038/nrm2673
Email
brenda.schulman@stjude.org
Published
2009-05-00
Epub
2009-00-08
Pages
319-31
Language
English
Region
England
NLM ID
100962782
PMCID
PMC2712597
Subset
IM
Grants
NIGMS NIH HHS · R01 GM054137-14 · United States
NIGMS NIH HHS · R01 GM077053-03 · United States
NIGMS NIH HHS · R01 GM069530-06 · United States
NIA NIH HHS · R03 AG022633-01 · United States
NIGMS NIH HHS · R01 GM070565-04 · United States
NIGMS NIH HHS · R01 GM077053 · United States
NIGMS NIH HHS · R01 GM069530 · United States
NIGMS NIH HHS · R01 GM054137 · United States
Howard Hughes Medical Institute · United States
NIA NIH HHS · R01 AG011085 · United States
NIGMS NIH HHS · R01 GM070565 · United States
NIA NIH HHS · R01 AG011085-16 · United States
External Links
PubMed source DOI: 10.1038/nrm2673 PMC Full Text View journal details
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