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PMID: 1989885 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

UBA 1: an essential yeast gene encoding ubiquitin-activating enzyme.

The EMBO journal ·Vol. 10 ·No. 1 ·1991-01-00 ·Pages 227-36

McGrath JP, Jentsch S, Varshavsky A

Abstract

All known functions of ubiquitin are mediated through its covalent attachment to other proteins. The post-translational formation of ubiquitin--protein conjugates is preceded by an ATP-requiring step in which the carboxyl terminus of ubiquitin is adenylated and subsequently joined, through a thiolester bond, to a cysteine residue in the ubiquitin-activating enzyme, also known as E1. We report the isolation and functional analysis of the gene (UBA1) for the ubiquitin-activating enzyme of the yeast Saccharomyces cerevisiae. UBA1 encodes a 114 kd protein whose amino acid sequence contains motifs characteristic of nucleotide-binding sites. Expression of catalytically active UBA1 protein in E. coli, which lacks the ubiquitin system, confirmed that the yeast UBA1 gene encodes a ubiquitin-activating enzyme. Deletion of the UBA1 gene is lethal, demonstrating that the formation of ubiquitin--protein conjugates is essential for cell viability.

Related Genes
Ch1N UBA1
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Escherichia coli/genetics Genes, Fungal Humans Ligases/genetics Molecular Sequence Data Oligonucleotide Probes Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics,physiology Sequence Homology, Nucleic Acid Spores, Fungal/physiology Transcription, Genetic Ubiquitin-Activating Enzymes Ubiquitin-Protein Ligases
Chemicals
Oligonucleotide Probes Ubiquitin-Protein Ligases Ligases Ubiquitin-Activating Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McGrath J P
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Jentsch S
Varshavsky A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-01-00
Pages
227-36
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452634
Subset
IM
Grants
NIDDK NIH HHS · DK39520 · United States
NIGMS NIH HHS · GM31530 · United States
Databases
GENBANK
M55400, M55401, M55402, M65224, S60919, S60920, X55386, X56507, X58577, X59082, X60368
External Links
PubMed source PMC Full Text View journal details
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