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PMID: 1986373 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 1 ·1991-01-01 ·Pages 258-62

Handley PM, Mueckler M, Siegel NR, Ciechanover A, Schwartz AL

Abstract

The ubiquitin-activating enzyme E1 catalyzes the first step in ubiquitin conjugation. We have cloned and sequenced the cDNA for human E1. This clone predicts a protein of 110,450 Da. Cys-194 lies within a region of identity to active-site Cys-88 of the ubiquitin carrier protein E2, suggesting a potential role for this region in enzymatic function of this protein. In addition, Cys-454 lies within a region of identity to the thiol ester consensus sequence of several proteins involved in thioester formation. Tissue distribution reveals a single 3.5-kilobase E1 message ubiquitous among tissues and cell lines.

MeSH Terms
Amino Acid Sequence Antibodies/isolation & purification Base Sequence Cloning, Molecular Erythrocytes/enzymology Female Gene Library Humans Ligases/blood,genetics,isolation & purification Molecular Sequence Data Organ Specificity Placenta/enzymology Pregnancy Protein Biosynthesis Transcription, Genetic Ubiquitin-Activating Enzymes Ubiquitin-Protein Ligases
Chemicals
Antibodies Ubiquitin-Protein Ligases Ligases Ubiquitin-Activating Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Handley P M
Department of Cell Biology, Washington University School of Medicine, St. Louis, MO.
Mueckler M
Siegel N R
Ciechanover A
Schwartz A L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.1.258
Published
1991-01-01
Pages
258-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50789
Subset
IM
Grants
NIDDK NIH HHS · DK38495 · United States
NIGMS NIH HHS · GM07805 · United States
NIGMS NIH HHS · GM38284 · United States
Databases
GENBANK
D90291, D90292, D90293, D90294, D90295, D90296, D90297, D90298, M58028, M62321
Corrections
ErratumIn
PMID: 1871145
External Links
PubMed source DOI: 10.1073/pnas.88.1.258 PMC Full Text View journal details
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