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PMID: 19199708 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).

Journal of proteome research ·Vol. 8 ·No. 3 ·2009-03-00 ·Pages 1304-14

Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR

Abstract

Human ductal saliva contributes over a thousand unique proteins to whole oral fluids. The mechanism by which most of these proteins are secreted by salivary glands remains to be determined. The present study used a mass spectrometry-based, shotgun proteomics approach to explore the possibility that a subset of the proteins found in saliva are derived from exosomes, membrane-bound vesicles of endosomal origin within multivesicular endosomes. Using MudPIT (multidimensional protein identification technology) mass spectrometry, we catalogued 491 proteins in the exosome fraction of human parotid saliva. Many of these proteins were previously observed in ductal saliva from parotid glands (265 proteins). Furthermore, 72 of the proteins in parotid exosomes overlap with those previously identified as urinary exosome proteins, proteins which are also frequently associated with exosomes from other tissues and cell types. Gene Ontology (GO) and KEGG pathway analyses found that cytosolic proteins comprise the largest category of proteins in parotid exosomes (43%), involved in such processes as phosphatidylinositol signaling system, calcium signaling pathway, inositol metabolism, protein export, and signal transduction, among others; whereas the integral plasma membrane proteins and associated/peripheral plasma membrane proteins (26%) were associated with extracellular matrix-receptor interaction, epithelial cell signaling, T-cell and B-cell receptor signaling, cytokine receptor interaction, and antigen processing and presentation, among other biological functions. In addition, these putative saliva exosomal proteins were linked to specific diseases (e.g., neurodegenerative disorders, prion disease, cancers, type I and II diabetes). Consequently, parotid glands secrete exosomes that reflect the metabolic and functional status of the gland and may also carry informative protein markers useful in the diagnosis and treatment of systemic diseases.

MeSH Terms
Endosomes/metabolism Exosomes/metabolism Humans Parotid Gland/metabolism Proteome/metabolism Saliva/metabolism
Chemicals
Proteome
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Gonzalez-Begne Mireya
Center for Oral Biology, University of Rochester Medical Center, Rochester, New York 14642, USA.
Lu Bingwen
Han Xuemei
Hagen Fred K
Hand Arthur R
Melvin James E
Yates John R
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Article Info
Journal
Journal of proteome research
Abbr.
J Proteome Res
ISSN
1535-3893
DOI
10.1021/pr800658c
Published
2009-03-00
Pages
1304-14
Language
English
Region
United States
NLM ID
101128775
PMCID
PMC2693447
Subset
IM
Grants
NIDCR NIH HHS · R01 DE09692 · United States
NIDCR NIH HHS · U01 DE016267 · United States
NIDCR NIH HHS · T32 DE07202 · United States
NCRR NIH HHS · P41 RR011823 · United States
NIDCR NIH HHS · R01 DE009692-13 · United States
NIDCR NIH HHS · UO1 DE016267 · United States
NIDCR NIH HHS · R01 DE08921 · United States
NCRR NIH HHS · P41 RR011823-09 · United States
NIDCR NIH HHS · R01 DE009692 · United States
NIDCR NIH HHS · R01 DE008921-14 · United States
NIDCR NIH HHS · U01 DE016267-04 · United States
NIDCR NIH HHS · R01 DE008921 · United States
NIDCR NIH HHS · T32 DE007202 · United States
External Links
PubMed source DOI: 10.1021/pr800658c PMC Full Text View journal details
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