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PMID: 19190889 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Development and genetic mapping of microsatellite markers from genome survey sequences in Brassica napus.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 118 ·No. 6 ·2009-04-00 ·Pages 1121-31

Cheng X, Xu J, Xia S, Gu J, Yang Y, Fu J, Qian X, Zhang S, Wu J, Liu K

Abstract

Microsatellite or simple sequence repeat (SSR) markers are routinely used for tagging genes and assessing genetic diversity. In spite of their importance, there are limited numbers of SSR markers available for Brassica crops. A total of 627 new SSR markers (designated BnGMS) were developed based on publicly available genome survey sequences and used to survey polymorphisms among six B. napus cultivars that serve as parents for established populations. Among these SSR markers, 591 (94.3%) successfully amplified at least one fragment and 434 (73.4%) detected polymorphism among the six B. napus cultivars. No correlation was observed between SSR motifs, repeat number or repeat length with polymorphism levels. A linkage map was constructed using 163 newly developed BnGMS marker loci and anchored with 164 public SSRs in a doubled haploid population. These new markers are evenly distributed over all linkage groups (LGs). Given that the majority of these SSRs are derived from bacterial artificial chromosome (BAC) end sequences, they will be useful in the assignment of their cognate BACs to LGs and facilitate the integration of physical maps with genetic maps for genome sequencing in B. napus.

MeSH Terms
Base Sequence Brassica napus/genetics Chromosome Mapping/methods Chromosomes, Plant Genetic Linkage Genome, Plant Microsatellite Repeats/genetics Molecular Sequence Data Polymorphism, Genetic
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Cheng Xiaomao
National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan, 430070, China.
Xu Jinsong
Xia Shu
Gu Jianxun
Yang Yuan
Fu Jie
Qian Xiaoju
Zhang Shunchang
Wu Jiangsheng
Liu Kede
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Article Info
Journal
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Abbr.
Theor Appl Genet
ISSN
1432-2242
DOI
10.1007/s00122-009-0967-8
Published
2009-04-00
Epub
2009-00-04
Pages
1121-31
Language
English
Region
Germany
NLM ID
0145600
Subset
IM
External Links
PubMed source DOI: 10.1007/s00122-009-0967-8 View journal details
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