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PMID: 17947401 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Construction of a sequence-tagged high-density genetic map of papaya for comparative structural and evolutionary genomics in brassicales.

Genetics ·Vol. 177 ·No. 4 ·2007-12-00 ·Pages 2481-91

Chen C, Yu Q, Hou S, Li Y, Eustice M, Skelton RL, Veatch O, Herdes RE, Diebold L, Saw J, Feng Y, Qian W, Bynum L, Wang L, Moore PH, Paull RE, Alam M, Ming R

Abstract

A high-density genetic map of papaya (Carica papaya L.) was constructed using microsatellite markers derived from BAC end sequences and whole-genome shot gun sequences. Fifty-four F(2) plants derived from varieties AU9 and SunUp were used for linkage mapping. A total of 707 markers, including 706 microsatellite loci and the morphological marker fruit flesh color, were mapped into nine major and three minor linkage groups. The resulting map spanned 1069.9 cM with an average distance of 1.5 cM between adjacent markers. This sequence-based microsatellite map resolved the very large linkage group 2 (LG 2) of the previous high-density map using amplified fragment length polymorphism markers. The nine major LGs of our map represent papaya's haploid nine chromosomes with LG 1 of the sex chromosome being the largest. This map validates the suppression of recombination at the male-specific region of the Y chromosome (MSY) mapped on LG 1 and at potential centromeric regions of other LGs. Segregation distortion was detected in a large region on LG 1 surrounding the MSY region due to the abortion of the YY genotype and in a region of LG6 due to an unknown cause. This high-density sequence-tagged genetic map is being used to integrate genetic and physical maps and to assign genome sequence scaffolds to papaya chromosomes. It provides a framework for comparative structural and evolutional genomic research in the order Brassicales.

MeSH Terms
Biological Evolution Brassicaceae/genetics Carica/genetics Chromosome Mapping/methods Chromosomes, Plant Genes, Plant Genetic Linkage Genome, Plant Microsatellite Repeats
Authors & Affiliations
18 authors, click to expand affiliations / ORCID
Chen Cuixia
Department of Tropical Plant and Soil Sciences, University of Hawaii, Honolulu, Hawaii 96822, USA.
Yu Qingyi
Hou Shaobin
Li Yingjun
Eustice Moriah
Skelton Rachel L
Veatch Olivia
Herdes Rachel E
Diebold Lauren
Saw Jimmy
Feng Yun
Qian Wubin
Bynum Lee
Wang Lei
Moore Paul H
Paull Robert E
Alam Maqsudul
Ming Ray
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.107.081463
Published
2007-12-00
Epub
2007-00-18
Pages
2481-91
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC2219497
Subset
IM
External Links
PubMed source DOI: 10.1534/genetics.107.081463 PMC Full Text View journal details
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