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PMID: 9475757 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A high-density rice genetic linkage map with 2275 markers using a single F2 population.

Genetics ·Vol. 148 ·No. 1 ·1998-01-00 ·Pages 479-94

Harushima Y, Yano M, Shomura A, Sato M, Shimano T, Kuboki Y, Yamamoto T, Lin SY, Antonio BA, Parco A, Kajiya H, Huang N, Yamamoto K, Nagamura Y, Kurata N, Khush GS, Sasaki T

Abstract

A 2275-marker genetic map of rice (Oryza sativa L.) covering 1521.6 cM in the Kosambi function has been constructed using 186 F2 plants from a single cross between the japonica variety Nipponbare and the indica variety Kasalath. The map provides the most detailed and informative genetic map of any plant. Centromere locations on 12 linkage groups were determined by dosage analysis of secondary and telotrisomics using > 130 DNA markers located on respective chromosome arms. A limited influence on meiotic recombination inhibition by the centromere in the genetic map was discussed. The main sources of the markers in this map were expressed sequence tag (EST) clones from Nipponbare callus, root, and shoot libraries. We mapped 1455 loci using ESTs; 615 of these loci showed significant similarities to known genes, including single-copy genes, family genes, and isozyme genes. The high-resolution genetic map permitted us to characterize meiotic recombinations in the whole genome. Positive interference of meiotic recombination was detected both by the distribution of recombination number per each chromosome and by the distribution of double crossover interval lengths.

MeSH Terms
Chromosome Mapping Genetic Markers/genetics Oryza/genetics Polymorphism, Genetic Polymorphism, Restriction Fragment Length
Chemicals
Genetic Markers
Authors & Affiliations
17 authors, click to expand affiliations / ORCID
Harushima Y
Rice Genome Research Program, National Institute of Agrobiological Resources/Institute of Society for Techno-Innovation of Agriculture, Forestry, and Fisheries, Tsukuba, Ibaraki, Japan.
Yano M
Shomura A
Sato M
Shimano T
Kuboki Y
Yamamoto T
Lin S Y
Antonio B A
Parco A
Kajiya H
Huang N
Yamamoto K
Nagamura Y
Kurata N
Khush G S
Sasaki T
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/148.1.479
Published
1998-01-00
Pages
479-94
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1459786
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/148.1.479 PMC Full Text View journal details
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