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PMID: 7987417 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Toward cataloguing all rice genes: large-scale sequencing of randomly chosen rice cDNAs from a callus cDNA library.

The Plant journal : for cell and molecular biology ·Vol. 6 ·No. 4 ·1994-10-00 ·Pages 615-24

Sasaki T, Song J, Koga-Ban Y, Matsui E, Fang F, Higo H, Nagasaki H, Hori M, Miya M, Murayama-Kayano E

Abstract

A large-scale sequence analysis of rice cDNA was performed for a library from rice callus cultured in a medium containing 1 p.p.m. of 2,4-dichlorophenoxyacetic acid. Random sequencing of 2778 cDNA clones generated 2259 non-redundant expressed sequence tags (ESTs). The strategy of sequencing cDNAs can yield quickly a large number of novel genes. After translation, 690 sequences showed a significant amino acid sequence similarity to sequences already known from PIR. The source of known proteins ranged from bacteria to human. In this report, the non-redundant set of 280 identified ESTs is analyzed in detail.

MeSH Terms

Animals DNA, Complementary Enzymes/genetics Gene Expression Gene Library Genes, Plant Humans Molecular Sequence Data Oryza/genetics Plant Proteins/genetics Sequence Homology, Nucleic Acid

Chemicals

DNA, Complementary Enzymes Plant Proteins

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Sasaki T

Rice Genome Research Program, National Institute of Agrobiological Resources/Institute of Society for Technoinnovation of Agriculture, Forestry and Fisheries, Ibaraki, Japan.

Song J

Koga-Ban Y

Matsui E

Fang F

Higo H

Nagasaki H

Hori M

Miya M

Murayama-Kayano E

Article Info

Journal

The Plant journal : for cell and molecular biology

Abbr.

Plant J

ISSN

0960-7412

DOI

10.1046/j.1365-313x.1994.6040615.x

Published

1994-10-00

Pages

615-24

Language

English

Region

England

NLM ID

9207397

Subset

Databases

GENBANK

D15075, D15076, D15077, D15078, D15079, D15080, D15081, D15082, D15083, D15084, D15085, D15086, D15087, D15088, D15089, D15090, D15091, D15092, D15093, D15094, D15095, D15096, D15097, D15098, D15099, D15100, D15101, D15102, D15103, D15104

External Links

PubMed source DOI: 10.1046/j.1365-313x.1994.6040615.x View journal details

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Toward cataloguing all rice genes: large-scale sequencing of randomly chosen rice cDNAs from a callus cDNA library.

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