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PMID: 17033785 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 114 ·No. 1 ·2006-12-00 ·Pages 67-80

Qiu D, Morgan C, Shi J, Long Y, Liu J, Li R, Zhuang X, Wang Y, Tan X, Dietrich E, Weihmann T, Everett C, Vanstraelen S, Beckett P, Fraser F, Trick M, Barnes S, Wilmer J, Schmidt R, Li J, Li D, Meng J, Bancroft I

Abstract

We have developed a new DH mapping population for oilseed rape, named TNDH, using genetically and phenotypically diverse parental lines. We used the population in the construction of a high stringency genetic linkage map, consisting of 277 loci, for use in quantitative genetic analysis. A proportion of the markers had been used previously in the construction of linkage maps for Brassica species, thus permitting the alignment of maps. The map includes 68 newly developed Sequence Tagged Site (STS) markers targeted to the homologues of defined genes of A. thaliana. The use of these markers permits the alignment of our linkage map with the A. thaliana genome sequence. An additional 74 loci (31 newly developed STS markers and 43 loci defined by SSR and RFLP markers that had previously been used in published linkage maps) were added to the map. These markers increased the resolution of alignment of the newly constructed linkage map with existing Brassica linkage maps and the A. thaliana genome sequence. We conducted field trials with the TNDH population at two sites, and over 2 years, and identified reproducible QTL for seed oil content and erucic acid content. The results provide new insights into the genetic control of seed oil and erucic acid content in oilseed rape, and demonstrate the utility of the linkage map and population.

MeSH Terms
Brassica napus/chemistry,genetics Chromosome Mapping Chromosomes, Plant Erucic Acids/analysis Genome, Plant Plant Oils/analysis Quantitative Trait Loci Seeds/chemistry
Chemicals
Erucic Acids Plant Oils erucic acid
Authors & Affiliations
23 authors, click to expand affiliations / ORCID
Qiu D
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 1 Shizishan, 430070, Wuhan, China.
Morgan C
Shi J
Long Y
Liu J
Li R
Zhuang X
Wang Y
Tan X
Dietrich E
Weihmann T
Everett C
Vanstraelen S
Beckett P
Fraser F
Trick M
Barnes S
Wilmer J
Schmidt R
Li J
Li D
Meng J
Bancroft I
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33 references, click to expand
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Article Info
Journal
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Abbr.
Theor Appl Genet
ISSN
0040-5752
DOI
10.1007/s00122-006-0411-2
Published
2006-12-00
Epub
2006-00-11
Pages
67-80
Language
English
Region
Germany
NLM ID
0145600
Subset
IM
External Links
PubMed source DOI: 10.1007/s00122-006-0411-2 View journal details
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