Home LiteratureArticle Details
PMID: 19188377 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

blaCTX-M genes in escherichia coli strains from Croatian Hospitals are located in new (blaCTX-M-3a) and widely spread (blaCTX-M-3a and blaCTX-M-15) genetic structures.

Antimicrobial agents and chemotherapy ·Vol. 53 ·No. 4 ·2009-04-00 ·Pages 1630-5

Literacka E, Bedenic B, Baraniak A, Fiett J, Tonkic M, Jajic-Bencic I, Gniadkowski M

Abstract

CTX-M-producing Escherichia coli isolates from three Croatian hospitals were analyzed. All bla(CTX-M-15) genes and one bla(CTX-M-3a) gene resided in widely spread ISEcp1 transposition modules, but other bla(CTX-M-3a) genes were in a new configuration with two IS26 copies, indicating a new event of gene mobilization from a Kluyvera ascorbata genome. The study confirmed the role of the E. coli ST131 clonal group with IncFII-type plasmids in the spread of bla(CTX-M-15) and of IncL/M pCTX-M3-type plasmids in the dissemination of bla(CTX-M-3a).

MeSH Terms
Base Sequence DNA Transposable Elements Escherichia coli/drug effects,enzymology,genetics Molecular Sequence Data Plasmids Polymerase Chain Reaction beta-Lactamases/genetics
Chemicals
DNA Transposable Elements beta-lactamase CTX-M-15 beta-lactamase CTX-M-3 beta-Lactamases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Literacka Elzbieta
National Medicines Institute, Warsaw, Poland.
Bedenic Branka
Baraniak Anna
Fiett Janusz
Tonkic Marija
Jajic-Bencic Ines
Gniadkowski Marek
References (38)
38 references, click to expand
  1. Identification of plasmids by PCR-based replicon typing.
    J Microbiol Methods. 2005 Dec;63(3):219-28 PMID: 15935499
  2. Replicon typing of plasmids carrying CTX-M or CMY beta-lactamases circulating among Salmonella and Escherichia coli isolates.
    Antimicrob Agents Chemother. 2006 Sep;50(9):3203-6 PMID: 16940132
  3. Direct sequencing of the amplified structural gene and promoter for the extended-broad-spectrum beta-lactamase TEM-9 (RHH-1) of Klebsiella pneumoniae.
    Plasmid. 1990 Jan;23(1):27-34 PMID: 2161546
  4. Growing group of extended-spectrum beta-lactamases: the CTX-M enzymes.
    Antimicrob Agents Chemother. 2004 Jan;48(1):1-14 PMID: 14693512
  5. Prevalence and spread of extended-spectrum beta-lactamase-producing Enterobacteriaceae in Europe.
    Clin Microbiol Infect. 2008 Jan;14 Suppl 1:144-53 PMID: 18154538
  6. Emergence of the quinolone resistance-mediating gene aac(6')-Ib-cr in extended-spectrum-beta-lactamase-producing Klebsiella isolates collected in Slovenia between 2000 and 2005.
    Antimicrob Agents Chemother. 2007 Nov;51(11):4171-3 PMID: 17846136
  7. Dissemination of clonally related Escherichia coli strains expressing extended-spectrum beta-lactamase CTX-M-15.
    Emerg Infect Dis. 2008 Feb;14(2):195-200 PMID: 18258110
  8. Complete nucleotide sequence of the pCTX-M3 plasmid and its involvement in spread of the extended-spectrum beta-lactamase gene blaCTX-M-3.
    Antimicrob Agents Chemother. 2007 Nov;51(11):3789-95 PMID: 17698626
  9. Ceftazidime-hydrolysing CTX-M-15 extended-spectrum beta-lactamase (ESBL) in Poland.
    J Antimicrob Chemother. 2002 Sep;50(3):393-6 PMID: 12205064
  10. The spread of CTX-M-type extended-spectrum beta-lactamases.
    Clin Microbiol Infect. 2008 Jan;14 Suppl 1:33-41 PMID: 18154526
  11. Extended broad-spectrum beta-lactamases conferring transferable resistance to newer beta-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns.
    Rev Infect Dis. 1988 Jul-Aug;10(4):867-78 PMID: 3263690
  12. First report of CTX-M extended-spectrum beta-lactamase-producing isolates from croatia.
    J Chemother. 2007 Feb;19(1):97-100 PMID: 17309859
  13. Cefotaxime-resistant Enterobacteriaceae isolates from a hospital in Warsaw, Poland: identification of a new CTX-M-3 cefotaxime-hydrolyzing beta-lactamase that is closely related to the CTX-M-1/MEN-1 enzyme.
    Antimicrob Agents Chemother. 1998 Apr;42(4):827-32 PMID: 9559791
  14. A new plasmidic cefotaximase in a clinical isolate of Escherichia coli.
    Infection. 1990 Sep-Oct;18(5):294-8 PMID: 2276823
  15. CTX-M: changing the face of ESBLs in Europe.
    J Antimicrob Chemother. 2007 Feb;59(2):165-74 PMID: 17158117
  16. In vitro analysis of ISEcp1B-mediated mobilization of naturally occurring beta-lactamase gene blaCTX-M of Kluyvera ascorbata.
    Antimicrob Agents Chemother. 2006 Apr;50(4):1282-6 PMID: 16569841
  17. Fluoroquinolone-modifying enzyme: a new adaptation of a common aminoglycoside acetyltransferase.
    Nat Med. 2006 Jan;12(1):83-8 PMID: 16369542
  18. Countrywide spread of CTX-M-3 extended-spectrum beta-lactamase-producing microorganisms of the family Enterobacteriaceae in Poland.
    Antimicrob Agents Chemother. 2002 Jan;46(1):151-9 PMID: 11751126
  19. Community and hospital spread of Escherichia coli producing CTX-M extended-spectrum beta-lactamases in the UK.
    J Antimicrob Chemother. 2004 Oct;54(4):735-43 PMID: 15347638
  20. Dominance of blaCTX-M within an Australian extended-spectrum beta-lactamase gene pool.
    Antimicrob Agents Chemother. 2008 Nov;52(11):4198-202 PMID: 18725449
  21. Clonal dissemination of a CTX-M-15 beta-lactamase-producing Escherichia coli strain in the Paris area, Tunis, and Bangui.
    Antimicrob Agents Chemother. 2006 Jul;50(7):2433-8 PMID: 16801423
  22. High-copy-number and low-copy-number plasmid vectors for lacZ alpha-complementation and chloramphenicol- or kanamycin-resistance selection.
    Gene. 1987;61(1):63-74 PMID: 3327753
  23. Complete nucleotide sequence of a 92-kilobase plasmid harboring the CTX-M-15 extended-spectrum beta-lactamase involved in an outbreak in long-term-care facilities in Toronto, Canada.
    Antimicrob Agents Chemother. 2004 Oct;48(10):3758-64 PMID: 15388431
  24. DNA sequence analysis of the genetic environment of various blaCTX-M genes.
    J Antimicrob Chemother. 2006 Jan;57(1):14-23 PMID: 16291869
  25. Characterization of clinical isolates of Klebsiella pneumoniae from 19 laboratories using the National Committee for Clinical Laboratory Standards extended-spectrum beta-lactamase detection methods.
    J Clin Microbiol. 2001 Aug;39(8):2864-72 PMID: 11474005
  26. CTX-M-10 linked to a phage-related element is widely disseminated among Enterobacteriaceae in a Spanish hospital.
    Antimicrob Agents Chemother. 2005 Apr;49(4):1567-71 PMID: 15793141
  27. Genetic support of extended-spectrum beta-lactamases.
    Clin Microbiol Infect. 2008 Jan;14 Suppl 1:75-81 PMID: 18154530
  28. Emergence of ArmA and RmtB aminoglycoside resistance 16S rRNA methylases in Belgium.
    J Antimicrob Chemother. 2007 Mar;59(3):459-64 PMID: 17224412
  29. Rapid and simple determination of the Escherichia coli phylogenetic group.
    Appl Environ Microbiol. 2000 Oct;66(10):4555-8 PMID: 11010916
  30. Sex and virulence in Escherichia coli: an evolutionary perspective.
    Mol Microbiol. 2006 Jun;60(5):1136-51 PMID: 16689791
  31. Nosocomial spread of armA-mediated high-level aminoglycoside resistance in Enterobacteriaceae isolates producing CTX-M-3 beta-lactamase in a cancer hospital in Bulgaria.
    J Chemother. 2008 Oct;20(5):593-9 PMID: 19028622
  32. High rate of mobilization for blaCTX-Ms.
    Emerg Infect Dis. 2008 Mar;14(3):423-8 PMID: 18325257
  33. Chromosome-encoded CTX-M-3 from Kluyvera ascorbata: a possible origin of plasmid-borne CTX-M-1-derived cefotaximases.
    Antimicrob Agents Chemother. 2004 Dec;48(12):4895-7 PMID: 15561876
  34. Comparative genomics of multidrug resistance in Acinetobacter baumannii.
    PLoS Genet. 2006 Jan;2(1):e7 PMID: 16415984
  35. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing.
    J Clin Microbiol. 1995 Sep;33(9):2233-9 PMID: 7494007
  36. Relatedness of chromosomal and plasmid DNAs of Erwinia pyrifoliae and Erwinia amylovora.
    Appl Environ Microbiol. 2002 Dec;68(12):6182-92 PMID: 12450843
  37. Plasmid-mediated extended-spectrum beta-lactamase (CTX-M-3 like) from India and gene association with insertion sequence ISEcp1.
    FEMS Microbiol Lett. 2001 Jul 24;201(2):237-41 PMID: 11470367
  38. Emergence and dissemination of Enterobacteriaceae isolates producing CTX-M-1-like enzymes in Spain are associated with IncFII (CTX-M-15) and broad-host-range (CTX-M-1, -3, and -32) plasmids.
    Antimicrob Agents Chemother. 2007 Feb;51(2):796-9 PMID: 17145793
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
1098-6596
DOI
10.1128/AAC.01431-08
Published
2009-04-00
Epub
2009-00-02
Pages
1630-5
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC2663074
Subset
IM
Databases
GENBANK
FM213371
External Links
PubMed source DOI: 10.1128/AAC.01431-08 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2