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PMID: 19132085 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Arabidopsis CULLIN3 genes regulate primary root growth and patterning by ethylene-dependent and -independent mechanisms.

PLoS genetics ·Vol. 5 ·No. 1 ·2009-01-00 ·Pages e1000328

Thomann A, Lechner E, Hansen M, Dumbliauskas E, Parmentier Y, Kieber J, Scheres B, Genschik P

Abstract

CULLIN3 (CUL3) together with BTB-domain proteins form a class of Cullin-RING ubiquitin ligases (called CRL3s) that control the rapid and selective degradation of important regulatory proteins in all eukaryotes. Here, we report that in the model plant Arabidopsis thaliana, CUL3 regulates plant growth and development, not only during embryogenesis but also at post-embryonic stages. First, we show that CUL3 modulates the emission of ethylene, a gaseous plant hormone that is an important growth regulator. A CUL3 hypomorphic mutant accumulates ACS5, the rate-limiting enzyme in ethylene biosynthesis and as a consequence exhibits a constitutive ethylene response. Second, we provide evidence that CUL3 regulates primary root growth by a novel ethylene-dependant pathway. In particular, we show that CUL3 knockdown inhibits primary root growth by reducing root meristem size and cell number. This phenotype is suppressed by ethylene-insensitive or resistant mutations. Finally, we identify a function of CUL3 in distal root patterning, by a mechanism that is independent of ethylene. Thus, our work highlights that CUL3 is essential for the normal division and organisation of the root stem cell niche and columella root cap cells.

MeSH Terms
Arabidopsis/genetics,growth & development,metabolism Arabidopsis Proteins/genetics,metabolism Carrier Proteins/genetics,physiology Cell Line Cotyledon/growth & development Cullin Proteins Ethylenes/metabolism Gene Expression Regulation, Plant Lyases/genetics,metabolism Mutation Oligonucleotide Array Sequence Analysis Plant Roots/anatomy & histology,genetics,growth & development
Chemicals
AT1G26830 protein, Arabidopsis Arabidopsis Proteins Carrier Proteins Cullin Proteins Ethylenes PIN7 protein, Arabidopsis cullin 3B protein, Arabidopsis ethylene Lyases 1-aminocyclopropanecarboxylate synthase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Thomann Alexis
ZMBP-Developmental Genetics, Universität Tübingen, Tübingen, Germany.
Lechner Esther
Hansen Maureen
Dumbliauskas Eva
Parmentier Yves
Kieber Joe
Scheres Ben
Genschik Pascal
Conflict of Interest

The authors have declared that no competing interests exist.

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Article Info
Journal
PLoS genetics
Abbr.
PLoS Genet
ISSN
1553-7404
DOI
10.1371/journal.pgen.1000328
Published
2009-01-00
Epub
2009-00-09
Pages
e1000328
Language
English
Region
United States
NLM ID
101239074
PMCID
PMC2607017
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pgen.1000328 PMC Full Text View journal details
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