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PMID: 9539813 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Recessive and dominant mutations in the ethylene biosynthetic gene ACS5 of Arabidopsis confer cytokinin insensitivity and ethylene overproduction, respectively.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 8 ·1998-04-14 ·Pages 4766-71

Vogel JP, Woeste KE, Theologis A, Kieber JJ

Abstract

We identified a set of cytokinin-insensitive mutants by using a screen based on the ethylene-mediated triple response observed after treatment with low levels of cytokinins. One group of these mutants disrupts ACS5, a member of the Arabidopsis gene family that encodes 1-aminocyclopropane-1-carboxylate synthase, the first enzyme in ethylene biosynthesis. The ACS5 isoform is mainly responsible for the sustained rise in ethylene biosynthesis observed in response to low levels of cytokinin and appears to be regulated primarily by a posttranscriptional mechanism. Furthermore, the dominant ethylene-overproducing mutant eto2 was found to be the result of an alteration of the carboxy terminus of ACS5, suggesting that this domain acts as a negative regulator of ACS5 function.

Keywords
Non-programmatic
MeSH Terms
Arabidopsis/enzymology,genetics,physiology Crosses, Genetic Cytokinins/pharmacology DNA Primers Ethylenes/metabolism Gene Expression Regulation, Plant/drug effects Genes, Dominant Genes, Recessive Hypocotyl Lyases/biosynthesis,genetics Polymerase Chain Reaction RNA, Messenger/biosynthesis Transcription, Genetic
Chemicals
Cytokinins DNA Primers Ethylenes RNA, Messenger ethylene Lyases 1-aminocyclopropanecarboxylate synthase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Vogel J P
Department of Biological Sciences, Laboratory for Molecular Biology, University of Illinois, Chicago, IL 60607, USA.
Woeste K E
Theologis A
Kieber J J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.8.4766
Published
1998-04-14
Pages
4766-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC22565
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.95.8.4766 PMC Full Text View journal details
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