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PMID: 19054391 Published · ppublish English Journal Article Research Support, N.I.H., Intramural

STAM adaptor proteins interact with COPII complexes and function in ER-to-Golgi trafficking.

Traffic (Copenhagen, Denmark) ·Vol. 10 ·No. 2 ·2009-02-00 ·Pages 201-17

Rismanchi N, Puertollano R, Blackstone C

Abstract

Signal-transducing adaptor molecules (STAMs) are involved in growth factor and cytokine signaling as well as receptor degradation, and they form complexes with a number of endocytic proteins, including Hrs and Eps15. In this study, we demonstrate that STAM proteins also localize prominently to early exocytic compartments and profoundly regulate Golgi morphology. Upon STAM overexpression in cells, the Golgi apparatus becomes extensively fragmented and dispersed, but when STAMs are depleted, the Golgi becomes highly condensed. Under both scenarios, vesicular stomatitis virus G protein-green fluorescent protein trafficking to the plasma membrane is markedly inhibited, and recovery of Golgi morphology after Brefeldin A treatment is substantially impaired in STAM-depleted cells. Furthermore, STAM proteins interact with coat protein II (COPII) proteins, probably at endoplasmic reticulum (ER) exit sites, and Sar1 activity is required to maintain the localization of STAMs at discrete sites. Thus, in addition to their roles in signaling and endocytosis, STAMs function prominently in ER-to-Golgi trafficking, most likely through direct interactions with the COPII complex.

MeSH Terms
Adaptor Proteins, Signal Transducing/genetics,metabolism COP-Coated Vesicles/metabolism,ultrastructure Endoplasmic Reticulum/metabolism,ultrastructure Endosomal Sorting Complexes Required for Transport Exocytosis GTP-Binding Proteins/metabolism Gene Deletion Gene Expression Regulation Golgi Apparatus/metabolism,ultrastructure HeLa Cells Humans Microscopy, Electron Phenotype Phosphoproteins/genetics,metabolism Protein Binding Protein Transport RNA, Small Interfering/genetics Time Factors Vesicular Transport Proteins/genetics,metabolism
Chemicals
Adaptor Proteins, Signal Transducing Endosomal Sorting Complexes Required for Transport Phosphoproteins RNA, Small Interfering STAM protein, human STAM2 protein, human Vesicular Transport Proteins GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rismanchi Neggy
Cellular Neurology Unit, Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
Puertollano Rosa
Blackstone Craig
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Article Info
Journal
Traffic (Copenhagen, Denmark)
Abbr.
Traffic
ISSN
1600-0854
DOI
10.1111/j.1600-0854.2008.00856.x
Published
2009-02-00
Epub
2008-00-18
Pages
201-17
Language
English
Region
England
NLM ID
100939340
PMCID
PMC2694054
Subset
IM
Grants
Intramural NIH HHS · Z01 NS002992-06 · United States
External Links
PubMed source DOI: 10.1111/j.1600-0854.2008.00856.x PMC Full Text View journal details
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