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PMID: 16880271 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

mBet3p is required for homotypic COPII vesicle tethering in mammalian cells.

The Journal of cell biology ·Vol. 174 ·No. 3 ·2006-07-31 ·Pages 359-68

Yu S, Satoh A, Pypaert M, Mullen K, Hay JC, Ferro-Novick S

Abstract

TRAPPI is a large complex that mediates the tethering of COPII vesicles to the Golgi (heterotypic tethering) in the yeast Saccharomyces cerevisiae. In mammalian cells, COPII vesicles derived from the transitional endoplasmic reticulum (tER) do not tether directly to the Golgi, instead, they appear to tether to each other (homotypic tethering) to form vesicular tubular clusters (VTCs). We show that mammalian Bet3p (mBet3p), which is the most highly conserved TRAPP subunit, resides on the tER and adjacent VTCs. The inactivation of mBet3p results in the accumulation of cargo in membranes that colocalize with the COPII coat. Furthermore, using an assay that reconstitutes VTC biogenesis in vitro, we demonstrate that mBet3p is required for the tethering and fusion of COPII vesicles to each other. Consistent with the proposal that mBet3p is required for VTC biogenesis, we find that ERGIC-53 (VTC marker) and Golgi architecture are disrupted in siRNA-treated mBet3p-depleted cells. These findings imply that the TRAPPI complex is essential for VTC biogenesis.

MeSH Terms
Animals Brefeldin A/pharmacology COP-Coated Vesicles/metabolism COS Cells Carrier Proteins/metabolism Chlorocebus aethiops Endoplasmic Reticulum/ultrastructure Golgi Apparatus/ultrastructure HeLa Cells Humans Protein Transport/drug effects Vesicular Transport Proteins/metabolism
Chemicals
Carrier Proteins SEC31A protein, human SEC31B protein, human TRAPPC3 protein, human Vesicular Transport Proteins Brefeldin A
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Yu Sidney
Howard Hughes Medical Institute and Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06519, USA.
Satoh Ayano
Pypaert Marc
Mullen Karl
Hay Jesse C
Ferro-Novick Susan
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200603044
Published
2006-07-31
Pages
359-68
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2064232
Subset
IM
Grants
NIGMS NIH HHS · R01 GM059378 · United States
NIGMS NIH HHS · GM59378 · United States
External Links
PubMed source DOI: 10.1083/jcb.200603044 PMC Full Text View journal details
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