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PMID: 1900939 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The transcriptional regulator LevR of Bacillus subtilis has domains homologous to both sigma 54- and phosphotransferase system-dependent regulators.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 6 ·1991-03-15 ·Pages 2212-6

Débarbouillé M, Martin-Verstraete I, Klier A, Rapoport G

Abstract

The regulatory gene levR of the levanase operon of Bacillus subtilis was cloned and sequenced. It encodes a polypeptide of Mr 106,064 with two domains homologous to members of two families of bacterial activators. One domain in LevR is homologous with one region of bacterial regulators including SacT and SacY of B. subtilis and BglG from Escherichia coli. Another domain of LevR is homologous to one part of the central domain of NifA and NtrC, which control nitrogen assimilation in Gram-negative bacteria. The levanase promoter contains two regions almost identical to the -12, -24 consensus regions present in sigma 54-dependent promoters. The expression of the levanase operon in E. coli was strongly dependent on sigma 54. Taken together, these results suggest that the operon is expressed from a -12, -24 promoter regulated by a sigma 54-like-dependent system in B. subtilis.

MeSH Terms
Amino Acid Sequence Bacillus subtilis/enzymology,genetics Bacterial Proteins/genetics Base Sequence Cloning, Molecular DNA, Bacterial/genetics Escherichia coli/genetics Genes, Regulator Glycoside Hydrolases/genetics Molecular Sequence Data Oligonucleotide Probes Operon Polymerase Chain Reaction Promoter Regions, Genetic Recombinant Fusion Proteins/metabolism Restriction Mapping Sequence Homology, Nucleic Acid Transcription Factors/genetics beta-Galactosidase/genetics,metabolism
Chemicals
Bacterial Proteins DNA, Bacterial Oligonucleotide Probes Recombinant Fusion Proteins Transcription Factors LevR protein, Bacillus subtilis Glycoside Hydrolases beta-Galactosidase levanase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Débarbouillé M
Unité de Biochimie Microbienne, URA 1300 du Centre National de la Recherche Scientifique, Paris, France.
Martin-Verstraete I
Klier A
Rapoport G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.6.2212
Published
1991-03-15
Pages
2212-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51200
Subset
IM
Databases
GENBANK
M58062, M58063, M58064, M58065, M58066, M58067, M59767, M59768, M60105, M61111
External Links
PubMed source DOI: 10.1073/pnas.88.6.2212 PMC Full Text View journal details
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