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PMID: 1900269 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

High-level expression of human c-jun gene causes cellular transformation of chicken embryo fibroblasts.

Japanese journal of cancer research : Gann ·Vol. 82 ·No. 1 ·1991-01-00 ·Pages 58-64

Suzuki T, Hashimoto Y, Okuno H, Sato H, Nishina H, Iba H

Abstract

To analyze the transforming activity of c-jun, a Rous sarcoma virus (RSV) variant that carries human c-jun instead of v-src (JH1) was constructed. After infection onto chicken embryo fibroblasts (CEF), JH1 formed foci with a titer comparable to that of wild-type RSV, and the infected cells grew in soft agar, indicating that the human c-jun gene has transforming potential, like the v-jun gene. The expression of Fra-2, one of the recently isolated Fos-related antigens, but not Fos was detected in both JH1-infected CEF and CEF infected with the control retrovirus vector (DS3). Gel shift analysis using nuclear extracts from DS3-infected CEF revealed that the Fra-2/Jun complex contributes to the basal level of AP-1 DNA binding activity. A similar activity was detected in JH1-infected CEF, but these cells have an additional AP-1 binding activity derived from Jun homodimers that seems to play important roles in the cellular transformation.

MeSH Terms
Animals Chick Embryo DNA Probes DNA-Binding Proteins/genetics Fibroblasts Humans Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-jun Transcription Factors/genetics Transfection Transformation, Genetic/genetics
Chemicals
DNA Probes DNA-Binding Proteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-jun Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Suzuki T
Department of Tumor Virus Research, University of Tokyo.
Hashimoto Y
Okuno H
Sato H
Nishina H
Iba H
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28 references, click to expand
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Article Info
Journal
Japanese journal of cancer research : Gann
Abbr.
Jpn J Cancer Res
ISSN
0910-5050
DOI
10.1111/j.1349-7006.1991.tb01746.x
Published
1991-01-00
Pages
58-64
Language
English
Region
Japan
NLM ID
8509412
PMCID
PMC5918208
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1349-7006.1991.tb01746.x PMC Full Text
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