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PMID: 1900061 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Purification and characterization of major antigens from a Mycobacterium bovis culture filtrate.

Infection and immunity ·Vol. 59 ·No. 3 ·1991-03-00 ·Pages 800-7

Fifis T, Costopoulos C, Radford AJ, Bacic A, Wood PR

Abstract

Ten major antigens from Mycobacterium bovis culture filtrate of 39, 32, 30, 25, 24, 22 (a and b forms), 19, 15, and 12 kDa have been purified and characterized by classical physicochemical methods. With monoclonal antibodies and/or N-terminal amino acid sequencing data, it was found that the antigens of 32, 30, 24, 22 (a), 19, and 12 kDa are related to M. bovis or M. tuberculosis antigens P32, MPB59, MPB64, MPB70, 19 kDa, and 12 kDa, respectively. The 39-, 25-, 22 (b)-, and 19-kDa antigens showed concanavalin A-binding properties and were positive in a glycan detection test, suggesting that they are glycoproteins. The 25- and 22 (b)-kDa proteins were found to be glycosylated forms of MPB70.

MeSH Terms
Amino Acid Sequence Antibodies, Monoclonal Antigens, Bacterial/chemistry,immunology,isolation & purification Carbohydrates/isolation & purification Chromatography, Gel Chromatography, Ion Exchange Concanavalin A Cross Reactions/immunology Electrophoresis, Polyacrylamide Gel Glycosylation Immunoblotting Molecular Sequence Data Monosaccharides/isolation & purification Mycobacterium bovis/immunology Mycobacterium tuberculosis/immunology
Chemicals
Antibodies, Monoclonal Antigens, Bacterial Carbohydrates Monosaccharides Concanavalin A
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fifis T
Commonwealth Scientific and Industrial Research Organisation, Division of Animal Health, Parkville, Victoria Australia.
Costopoulos C
Radford A J
Bacic A
Wood P R
References (36)
36 references, click to expand
  1. Complete nucleotide sequence of immunogenic protein MPB70 from Mycobacterium bovis BCG.
    FEMS Microbiol Lett. 1989 Apr;49(2-3):273-6 PMID: 2663636
  2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  3. Purification, characterization and identification of a 32 kDa protein antigen of Mycobacterium bovis BCG.
    Microb Pathog. 1987 May;2(5):351-66 PMID: 3148811
  4. Cloning and expression of the Mycobacterium bovis BCG gene for extracellular alpha antigen.
    J Bacteriol. 1988 Sep;170(9):3847-54 PMID: 2842287
  5. Immunological activity of a 14-kilodalton recombinant protein of Mycobacterium tuberculosis H37Rv.
    Infect Immun. 1987 Dec;55(12):3149-54 PMID: 3119499
  6. The antigens of Mycobacterium tuberculosis, H37Rv, studied by crossed immunoelectrophoresis. Comparison with a reference system for Mycobacterium bovis, BCG.
    Scand J Immunol. 1988 Feb;27(2):223-39 PMID: 3124264
  7. Immunogenic protein MPB57 from Mycobacterium bovis BCG: molecular cloning, nucleotide sequence and expression.
    FEBS Lett. 1988 Nov 21;240(1-2):115-7 PMID: 3056744
  8. Cloning and sequence analysis of the 10 kDa antigen gene of Mycobacterium tuberculosis.
    J Gen Microbiol. 1989 Apr;135(4):931-9 PMID: 2480990
  9. Identification, isolation and partial characterization of Mycobacterium tuberculosis glycoprotein antigens.
    Clin Exp Immunol. 1989 Sep;77(3):378-83 PMID: 2478323
  10. Structure of mycobacterial antigens.
    Br Med Bull. 1988 Jul;44(3):562-83 PMID: 2476193
  11. Production and characterization of monoclonal antibodies specific for Mycobacterium bovis.
    J Gen Microbiol. 1988 Sep;134(9):2599-604 PMID: 2474629
  12. Immunological activity of a 38-kilodalton protein purified from Mycobacterium tuberculosis.
    Infect Immun. 1986 Oct;54(1):177-83 PMID: 2428751
  13. Interspecies reactivity of five monoclonal antibodies to Mycobacterium tuberculosis as examined by immunoblotting and enzyme-linked immunosorbent assay.
    J Clin Microbiol. 1986 Mar;23(3):446-51 PMID: 2420818
  14. Cloning, sequence determination, and expression of a 32-kilodalton-protein gene of Mycobacterium tuberculosis.
    Infect Immun. 1989 Oct;57(10):3123-30 PMID: 2506131
  15. Nucleotide sequence of the 19 kDa antigen gene from Mycobacterium tuberculosis.
    Nucleic Acids Res. 1989 Feb 11;17(3):1249 PMID: 2493628
  16. Purification of a major Mycobacterium bovis antigen for the diagnosis of bovine tuberculosis.
    Scand J Immunol. 1989 Jan;29(1):91-101 PMID: 2646701
  17. Cloning and characterization of the gene for immunogenic protein MPB64 of Mycobacterium bovis BCG.
    Infect Immun. 1989 Jan;57(1):283-8 PMID: 2642468
  18. The chemical composition of immunoaffinity-purified Mycobacterium tuberculosis antigen 5.
    Am Rev Respir Dis. 1989 Jun;139(6):1566-7 PMID: 2729761
  19. A family of glycoinositol phospholipids from Leishmania major. Isolation, characterization, and antigenicity.
    J Biol Chem. 1989 Jan 15;264(2):757-66 PMID: 2910865
  20. Cloning of a species-specific antigen of Mycobacterium bovis.
    Infect Immun. 1988 Apr;56(4):921-5 PMID: 3278986
  21. Purification and characterization of a tuberculin-active substance from Mycobacterium bovis BCG.
    J Biochem. 1984 Aug;96(2):523-31 PMID: 6389525
  22. Immunological evaluation of a component isolated from Mycobacterium bovis BCG with a monoclonal antibody to M. bovis BCG.
    Infect Immun. 1984 Nov;46(2):519-25 PMID: 6389346
  23. Immunopurification of radiolabelled antigens of Mycobacterium leprae and Mycobacterium bovis (bacillus Calmette-Guerin) with monoclonal antibodies.
    Scand J Immunol. 1987 Aug;26(2):149-59 PMID: 3306906
  24. Characterization, sequence determination, and immunogenicity of a 64-kilodalton protein of Mycobacterium bovis BCG expressed in escherichia coli K-12.
    Infect Immun. 1987 Jun;55(6):1466-75 PMID: 3553003
  25. Purification, partial characterization, and identification of a skin-reactive protein antigen of Mycobacterium bovis BCG.
    Infect Immun. 1987 Jan;55(1):245-52 PMID: 3539805
  26. MPB59, a widely cross-reacting protein of Mycobacterium bovis BCG.
    Int Arch Allergy Appl Immunol. 1986;81(4):307-14 PMID: 3536756
  27. Purification and characterization of two protein antigens from the heterogeneous BCG85 complex in Mycobacterium bovis BCG.
    Int Arch Allergy Appl Immunol. 1986;81(4):298-306 PMID: 3536755
  28. Immunoreactivity of a 70 kD protein purified from Mycobacterium bovis Bacillus Calmette-Guerin by monoclonal antibody affinity chromatography.
    J Exp Med. 1986 Sep 1;164(3):695-708 PMID: 3528377
  29. Properties of proteins MPB64, MPB70, and MPB80 of Mycobacterium bovis BCG.
    Infect Immun. 1986 Apr;52(1):293-302 PMID: 3514457
  30. Production and partial characterization of monoclonal hybridoma antibodies to Mycobacterium tuberculosis.
    Acta Pathol Microbiol Immunol Scand C. 1985 Dec;93(6):265-72 PMID: 3939074
  31. Measurement of protein using bicinchoninic acid.
    Anal Biochem. 1985 Oct;150(1):76-85 PMID: 3843705
  32. A rapid and sensitive method for the analysis of carbohydrate components in glycoproteins using gas-liquid chromatography.
    Anal Biochem. 1982 Jul 1;123(2):336-41 PMID: 6181710
  33. Specificity of Mycobacterium tuberculosis antigen 5 determined with mouse monoclonal antibodies.
    Infect Immun. 1984 Jul;45(1):52-5 PMID: 6376360
  34. Immunobiology and species distribution of Mycobacterium tuberculosis antigen 5.
    Infect Immun. 1979 Apr;24(1):77-82 PMID: 110696
  35. The isolation by immunoabsorbent affinity chromatography and physicochemical characterization of Mycobacterium tuberculosis antigen 5.
    Am Rev Respir Dis. 1978 Mar;117(3):533-9 PMID: 415649
  36. Glycosyl-phosphatidylinositol moiety that anchors Trypanosoma brucei variant surface glycoprotein to the membrane.
    Science. 1988 Feb 12;239(4841 Pt 1):753-9 PMID: 3340856
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.3.800-807.1991
Published
1991-03-00
Pages
800-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258330
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.59.3.800-807.1991 PMC Full Text View journal details
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