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PMID: 3119499 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Immunological activity of a 14-kilodalton recombinant protein of Mycobacterium tuberculosis H37Rv.

Infection and immunity ·Vol. 55 ·No. 12 ·1987-12-00 ·Pages 3149-54

Kingston AE, Salgame PR, Mitchison NA, Colston MJ

Abstract

A 14-kilodalton peptide antigen from Mycobacterium tuberculosis was isolated from an Escherichia coli lambda gt 11 recombinant DNA clone and was identified by Western blotting (immunoblotting) with monoclonal antibody TB68. Immunization of mice and guinea pigs with the recombinant peptide (rTB68) induced in vitro lymphoproliferative responses in draining lymph node lymphocyte cultures as well as in vivo delayed-type hypersensitivity reactions. Moreover, rTB68 was found both to induce and to cross-react with Mycobacterium leprae immune lymphocytes, but did not generate protective effects against live M. leprae challenge in mice. These findings showed that a 14-kilodalton peptide which has been characterized as specific for M. tuberculosis on the basis of B-cell recognition was capable of generating cell-mediated immune responses and moreover contained T-cell epitopes which were cross-reactive with M. leprae antigens.

MeSH Terms
Antigens, Bacterial/genetics,immunology Bacterial Proteins/immunology Cross Reactions Hypersensitivity, Delayed/immunology Immunity, Cellular Immunization Lymphocyte Activation Molecular Weight Mycobacterium Infections/prevention & control Mycobacterium leprae/immunology Mycobacterium tuberculosis/immunology Recombinant Fusion Proteins/immunology Recombinant Proteins/immunology T-Lymphocytes/immunology
Chemicals
Antigens, Bacterial Bacterial Proteins Recombinant Fusion Proteins Recombinant Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kingston A E
Laboratory for Leprosy and Mycobacterial Research, National Institute for Medical Research, Mill Hill, London, United Kingdom.
Salgame P R
Mitchison N A
Colston M J
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.12.3149-3154.1987
Published
1987-12-00
Pages
3149-54
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260041
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.55.12.3149-3154.1987 PMC Full Text View journal details
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