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PMID: 18824477 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Yeast ribosomal protein L10 helps coordinate tRNA movement through the large subunit.

Nucleic acids research ·Vol. 36 ·No. 19 ·2008-11-00 ·Pages 6187-98

Petrov AN, Meskauskas A, Roshwalb SC, Dinman JD

Abstract

Yeast ribosomal protein L10 (E. coli L16) is located at the center of a topological nexus that connects many functional regions of the large subunit. This essential protein has previously been implicated in processes as diverse as ribosome biogenesis, translational fidelity and mRNA stability. Here, the inability to maintain the yeast Killer virus was used as a proxy for large subunit defects to identify a series of L10 mutants. These mapped to roughly four discrete regions of the protein. A detailed analysis of mutants located in the N-terminal 'hook' of L10, which inserts into the bulge of 25S rRNA helix 89, revealed strong effects on rRNA structure corresponding to the entire path taken by the tRNA 3' end as it moves through the large subunit during the elongation cycle. The mutant-induced structural changes are wide-ranging, affecting ribosome biogenesis, elongation factor binding, drug resistance/hypersensitivity, translational fidelity and virus maintenance. The importance of L10 as a potential transducer of information through the ribosome, and of a possible role of its N-terminal domain in switching between the pre- and post-translocational states are discussed.

MeSH Terms
Alleles Base Sequence Models, Molecular Molecular Sequence Data Mutation Peptide Chain Elongation, Translational Protein Biosynthesis RNA, Ribosomal/chemistry RNA, Transfer/chemistry,metabolism Ribosomal Protein L10 Ribosomal Proteins/chemistry,genetics Ribosome Subunits, Large, Eukaryotic/chemistry,metabolism Ribosomes/chemistry Saccharomyces cerevisiae/genetics,growth & development Saccharomyces cerevisiae Proteins/chemistry,genetics
Chemicals
RNA, Ribosomal Ribosomal Proteins Saccharomyces cerevisiae Proteins RNA, ribosomal, 25S RNA, Transfer
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Petrov Alexey N
Department of Cell Biology and Molecular Genetics, University of Maryland, 2135 Microbiology Building, College Park, MD 20742, USA.
Meskauskas Arturas
Roshwalb Sara C
Dinman Jonathan D
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkn643
Published
2008-11-00
Epub
2008-00-29
Pages
6187-98
Language
English
Region
England
NLM ID
0411011
PMCID
PMC2577338
Subset
IM
Grants
NIGMS NIH HHS · R01 GM058859 · United States
NIGMS NIH HHS · GM058859 · United States
External Links
PubMed source DOI: 10.1093/nar/gkn643 PMC Full Text View journal details
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