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PMID: 18701674 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Amino acid polymorphisms in strictly conserved domains of a P-type ATPase HMA5 are involved in the mechanism of copper tolerance variation in Arabidopsis.

Plant physiology ·Vol. 148 ·No. 2 ·2008-10-00 ·Pages 969-80

Kobayashi Y, Kuroda K, Kimura K, Southron-Francis JL, Furuzawa A, Kimura K, Iuchi S, Kobayashi M, Taylor GJ, Koyama H

Abstract

Copper (Cu) is an essential element in plant nutrition, but it inhibits the growth of roots at low concentrations. Accessions of Arabidopsis (Arabidopsis thaliana) vary in their tolerance to Cu. To understand the molecular mechanism of Cu tolerance in Arabidopsis, we performed quantitative trait locus (QTL) analysis and accession studies. One major QTL on chromosome 1 (QTL1) explained 52% of the phenotypic variation in Cu tolerance in roots in a Landsberg erecta/Cape Verde Islands (Ler/Cvi) recombinant inbred population. This QTL regulates Cu translocation capacity and involves a Cu-transporting P(1B-1)-type ATPase, HMA5. The Cvi allele carries two amino acid substitutions in comparison with the Ler allele and is less functional than the Ler allele in Cu tolerance when judged by complementation assays using a T-DNA insertion mutant. Complementation assays of the ccc2 mutant of yeast using chimeric HMA5 proteins revealed that N923T of the Cvi allele, which was identified in the tightly conserved domain N(x)(6)YN(x)(4)P (where the former asparagine was substituted by threonine), is a cause of dysfunction of the Cvi HMA5 allele. Another dysfunctional HMA5 allele was identified in Chisdra-2, which showed Cu sensitivity and low capacity of Cu translocation from roots to shoots. A unique amino acid substitution of Chisdra-2 was identified in another strictly conserved domain, CPC(x)(6)P, where the latter proline was replaced with leucine. These results indicate that a portion of the variation in Cu tolerance of Arabidopsis is regulated by the functional integrity of the Cu-translocating ATPase, HMA5, and in particular the amino acid sequence in several strictly conserved motifs.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Alleles Amino Acid Motifs Amino Acid Sequence Amino Acid Substitution Arabidopsis/genetics,metabolism Arabidopsis Proteins/genetics,metabolism Conserved Sequence Copper/metabolism DNA, Bacterial/genetics DNA, Plant/genetics Genes, Plant Genetic Complementation Test Genotype Molecular Sequence Data Mutagenesis, Insertional Phenotype Polymorphism, Genetic Quantitative Trait Loci Saccharomyces cerevisiae/genetics,metabolism
Chemicals
Arabidopsis Proteins DNA, Bacterial DNA, Plant T-DNA Copper Adenosine Triphosphatases
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Kobayashi Yuriko
Laboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
Kuroda Keishi
Kimura Keisuke
Southron-Francis Jennafer L
Furuzawa Aya
Kimura Kazuhiko
Iuchi Satoshi
Kobayashi Masatomo
Taylor Gregory J
Koyama Hiroyuki
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.108.119933
Published
2008-10-00
Epub
2008-00-13
Pages
969-80
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC2556842
Subset
IM
Databases
GENBANK
EU877765, EU877766, EU877767, EU877768, EU877769, EU877770, EU877771, EU877772, EU877773, EU877774, EU877775, EU877776, EU877777, EU877778, EU877779, EU877780, EU877781, EU877782, EU877783, EU877784, EU877785, EU877786, EU877787, EU877788, EU877789, EU877790, EU877791, EU877792, EU877793, EU877794, EU877795, EU877796, EU877797, EU877798, EU877799, EU877800, EU877801, EU877802, EU877803
External Links
PubMed source DOI: 10.1104/pp.108.119933 PMC Full Text View journal details
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