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PMID: 16126858 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A copper chaperone for superoxide dismutase that confers three types of copper/zinc superoxide dismutase activity in Arabidopsis.

Plant physiology ·Vol. 139 ·No. 1 ·2005-09-00 ·Pages 425-36

Chu CC, Lee WC, Guo WY, Pan SM, Chen LJ, Li HM, Jinn TL

Abstract

The copper chaperone for superoxide dismutase (CCS) has been identified as a key factor integrating copper into copper/zinc superoxide dismutase (CuZnSOD) in yeast (Saccharomyces cerevisiae) and mammals. In Arabidopsis (Arabidopsis thaliana), only one putative CCS gene (AtCCS, At1g12520) has been identified. The predicted AtCCS polypeptide contains three distinct domains: a central domain, flanked by an ATX1-like domain, and a C-terminal domain. The ATX1-like and C-terminal domains contain putative copper-binding motifs. We have investigated the function of this putative AtCCS gene and shown that a cDNA encoding the open reading frame predicted by The Arabidopsis Information Resource complemented only the cytosolic and peroxisomal CuZnSOD activities in the Atccs knockout mutant, which has lost all CuZnSOD activities. However, a longer AtCCS cDNA, as predicted by the Munich Information Centre for Protein Sequences and encoding an extra 66 amino acids at the N terminus, could restore all three, including the chloroplastic CuZnSOD activities in the Atccs mutant. The extra 66 amino acids were shown to direct the import of AtCCS into chloroplasts. Our results indicated that one AtCCS gene was responsible for the activation of all three types of CuZnSOD activity. In addition, a truncated AtCCS, containing only the central and C-terminal domains without the ATX1-like domain failed to restore any CuZnSOD activity in the Atccs mutant. This result indicates that the ATX1-like domain is essential for the copper chaperone function of AtCCS in planta.

MeSH Terms
Amino Acid Sequence Arabidopsis/enzymology,genetics,metabolism Arabidopsis Proteins/chemistry,genetics,metabolism Chloroplasts/metabolism Copper/metabolism Gene Deletion Gene Expression Regulation, Plant Genetic Complementation Test Molecular Chaperones/chemistry,genetics,metabolism Molecular Sequence Data Plant Leaves/metabolism Plants, Genetically Modified Protein Structure, Tertiary Sequence Homology, Amino Acid Superoxide Dismutase/classification,metabolism Zinc/metabolism
Chemicals
Arabidopsis Proteins CCS protein, Arabidopsis Molecular Chaperones Copper Superoxide Dismutase Zinc
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Chu Chiung-Chih
Department of Life Science and Institute of Plant Biology, National Taiwan University, Taipei.
Lee Wen-Chi
Guo Wen-Yu
Pan Shu-Mei
Chen Lih-Jen
Li Hsou-Min
Jinn Tsung-Luo
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.105.065284
Published
2005-09-00
Epub
2005-00-26
Pages
425-36
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1203391
Subset
IM
Databases
GENBANK
DQ003054, DQ003058
External Links
PubMed source DOI: 10.1104/pp.105.065284 PMC Full Text View journal details
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