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PMID: 11549763 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Leaf-specific upregulation of chloroplast translocon genes by a CCT motif-containing protein, CIA 2.

The Plant cell ·Vol. 13 ·No. 9 ·2001-09-00 ·Pages 2053-61

Sun CW, Chen LJ, Lin LC, Li HM

Abstract

Chloroplasts are a major destination of protein traffic within leaf cells. Protein import into chloroplasts is mediated by a set of translocon complexes at the chloroplast envelope. Current data indicate that the expression of translocon genes is regulated in a tissue-specific manner, possibly to accommodate the higher import demand of chloroplasts in leaves and the lower demand of plastids in other tissues. We have designed a transgene-based positive screen to isolate mutants disrupted in protein import into plastids. The first locus we isolated, CIA2, encodes a protein containing a motif conserved within the CCT family of transcription factors. Biochemical analysis indicates that CIA2 is responsible for specific upregulation of the translocon genes atToc33 and atToc75 in leaves. Identification of CIA2 provides new insights into the tissue-specific regulation of translocon gene expression.

MeSH Terms
Amino Acid Motifs Amino Acid Sequence Arabidopsis/genetics,metabolism,ultrastructure Arabidopsis Proteins/chemistry,genetics,metabolism Chloroplast Proteins Chloroplasts/genetics,metabolism,ultrastructure Cloning, Molecular DNA, Chloroplast/genetics Gene Expression Regulation, Plant Genes, Reporter Membrane Proteins/genetics,metabolism Molecular Sequence Data Mutation Nuclear Localization Signals Nuclear Proteins/chemistry,genetics,metabolism Organ Specificity Plant Leaves/genetics,metabolism,ultrastructure Plant Proteins Protein Precursors/genetics,metabolism Protein Transport RNA, Messenger/analysis,genetics RNA, Plant/analysis,genetics Reverse Transcriptase Polymerase Chain Reaction Transcription Factors/chemistry,genetics,metabolism Transgenes/genetics Up-Regulation
Chemicals
Arabidopsis Proteins CIA2 protein, Arabidopsis Chloroplast Proteins DNA, Chloroplast Membrane Proteins Nuclear Localization Signals Nuclear Proteins OEP75 protein precursor, plant Plant Proteins Protein Precursors RNA, Messenger RNA, Plant TOC75 protein, Arabidopsis Toc33 protein, Arabidopsis Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sun C W
Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan.
Chen L J
Lin L C
Li H M
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.010148
Published
2001-09-00
Pages
2053-61
Language
English
Region
England
NLM ID
9208688
PMCID
PMC139451
Subset
IM
Databases
GENBANK
AF272039, AF359387, AF359388, C81119, Y10555
External Links
PubMed source DOI: 10.1105/tpc.010148 PMC Full Text View journal details
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