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PMID: 12650623 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of a copper transporter family in Arabidopsis thaliana.

Plant molecular biology ·Vol. 51 ·No. 4 ·2003-03-00 ·Pages 577-87

Sancenón V, Puig S, Mira H, Thiele DJ, Peñarrubia L

Abstract

Despite copper ions being crucial in proteins participating in plant processes such as electron transport, free-radical elimination and hormone perception and signaling, very little is known about copper inward transport across plant membranes. In this work, a five-member family (COPT1-5) of putative Arabidopsis copper transporters is described. We ascertain the ability of these proteins to functionally complement and transport copper in the corresponding Saccharomyces cerevisiae high-affinity copper transport mutant. The specific expression pattern of the Arabidopsis COPT1-5 mRNA in different tissues was analyzed by RT-PCR. Although all members are ubiquitously expressed, differences in their relative abundance in roots, leaves, stem and flowers have been observed. Moreover, steady-state COPT1 and COPT2 mRNA levels, the members that are most efficacious in complementing the S. cerevisiae high-affinity copper transport mutant, are down-regulated under copper excess, consistent with a role for these proteins in copper transport in Arabidopsis cells.

MeSH Terms
Amino Acid Sequence Arabidopsis/drug effects,genetics Arabidopsis Proteins/genetics Cloning, Molecular Copper/metabolism,pharmacology Copper Transporter 1 DNA, Complementary/chemistry,genetics Gene Expression Regulation, Plant/genetics Genetic Complementation Test Membrane Transport Proteins/genetics Molecular Sequence Data Multigene Family/genetics Mutation Phylogeny RNA, Messenger/drug effects,genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid
Chemicals
Arabidopsis Proteins COPT1 protein, Arabidopsis Copper Transporter 1 DNA, Complementary Membrane Transport Proteins RNA, Messenger Copper
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sancenón Vicente
Departament de Bioquímica i Biologia Molecular, Universitat de València, Dr. Moliner 50, Burjassot, València, 46100 Spain.
Puig Sergi
Mira Helena
Thiele Dennis J
Peñarrubia Lola
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1022345507112
Published
2003-03-00
Pages
577-87
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Grants
NCI NIH HHS · 1R24CA 86307 · United States
NIGMS NIH HHS · GM41840 · United States
Databases
GENBANK
AF466370, AF466371, AF466372, AF466373, AF466374
External Links
PubMed source DOI: 10.1023/a:1022345507112 View journal details
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