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PMID: 186462 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Microtubule assembly in cultivated Greene melanoma cells is stimulated by dibutyryl adenosine 3':5'-cyclic monophosphate or cholera toxin.

The Journal of cell biology ·Vol. 71 ·No. 3 ·1976-12-00 ·Pages 735-48

DiPasquale AM, McGuire J, Moellmann G, Wasserman SJ

Abstract

Both dibutyryl cyclic AMP (DBcAMP) and cholera toxin promote the formation and elongation of processes of cultivated Greene hamster melanoma cells. The formation and maintenance of these processes, which contain many microtubules, are sensitive to colcemid and vinblastine. Tubulin was measured by [3H]colchicine binding and by acrylamide gel electrophoresis. We found that DBcAMP or cholera toxin increases the ratio of polymerized to unpolymerized tubulin but not the total amount of tubulin per cell. The sum of the lengths of microtubules per unit area was significantly greater in cells treated with DBcAMP than in control cells. Our findings support the hypothesis that cyclic AMP promotes the elongation of cell processes by stimulating the assembly of microtubules from existing tubulin.

MeSH Terms
Bacterial Toxins/pharmacology Bucladesine/pharmacology Cell Line Colchicine/pharmacology Cycloheximide/pharmacology Melanocyte-Stimulating Hormones/pharmacology Microtubules/drug effects Polymers Tubulin/analysis,metabolism Vibrio cholerae Vinblastine/pharmacology
Chemicals
Bacterial Toxins Polymers Tubulin Vinblastine Bucladesine Melanocyte-Stimulating Hormones Cycloheximide Colchicine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
DiPasquale A M
McGuire J
Moellmann G
Wasserman S J
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51 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.71.3.735
Published
1976-12-00
Pages
735-48
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2109777
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.71.3.735 PMC Full Text View journal details
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