Microtubule formation in vitro in solutions containing low calcium concentrations.

Science (New York, N.Y.) ·Vol. 177 ·No. 4054 ·1972-09-22 ·Pages 1104-5

Weisenberg RC

Abstract

Isolated rat brain tubulin can be repolymerized in vitro in solutions containing adenosine triphosphate or guanosine triphosphate, magnesium ions, and a good calcium chelator. The extreme sensitivity of tubulin to calcium ions explains the failure of previous efforts to obtain polymerization and suggests a possible mechanism for regulation of microtubule polymerization in vivo.

MeSH Terms

Adenosine Triphosphate/pharmacology Animals Brain/metabolism Calcium/pharmacology Calcium Chloride/pharmacology Chelating Agents/pharmacology Edetic Acid/pharmacology Guanosine Triphosphate/pharmacology In Vitro Techniques Magnesium Sulfate/pharmacology Microtubules/metabolism Polymers Rats Tissue Extracts/metabolism

Chemicals

Chelating Agents Polymers Tissue Extracts Magnesium Sulfate Guanosine Triphosphate Adenosine Triphosphate Edetic Acid Calcium Chloride Calcium

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Weisenberg R C

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.177.4054.1104

Published

1972-09-22

Pages

1104-5

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.177.4054.1104 View journal details

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Microtubule formation in vitro in solutions containing low calcium concentrations.

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