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PMID: 186449 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Fosfomycin resistance: selection method for internal and extended deletions of the phosphoenolpyruvate:sugar phosphotransferase genes of Salmonella typhimurium.

Journal of bacteriology ·Vol. 128 ·No. 3 ·1976-12-00 ·Pages 785-93

Cordaro JC, Melton T, Stratis JP, Atagün M, Gladding C, Hartman PE, Roseman S

Abstract

Selection for resistance to the antibiotic fosfomycin (FOS; L-cis 1,2-epoxypropylphosphonic acid, a structural analogue of phosphoenolpyruvate) was used to isolate mutants carrying internal and extended deletions of varying lengths within the ptsHI operon of Salmonella typhimurium. Strains carrying "tight" ptsI point mutations and all mutants in which some or all of the ptsI gene was deleted were FOS resistant. In contrast, strains carrying ptsH point mutations were sensitive to FOS. Resistance to FOS appeared to result indirectly from catabolite repression of an FOS transport system, probably the sn-glycerol-3-phosphate transport system. Resistant ptsI mutants became sensitive to FOS when grown on D-glucose-6-phosphate, which induces an alternate transport system for FOS, or when grown in the presence of cyclic adenosine 3',5'-monophosphate. A detailed fine-structure map of the pts gene region is presented.

MeSH Terms

Anti-Bacterial Agents/pharmacology Biological Transport, Active Chromosome Mapping Chromosomes, Bacterial Cyclic AMP/pharmacology Drug Resistance, Microbial Fosfomycin/metabolism,pharmacology Mutation Operon Phosphotransferases/biosynthesis Salmonella typhimurium/drug effects,enzymology

Chemicals

Anti-Bacterial Agents Fosfomycin Cyclic AMP Phosphotransferases

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Cordaro J C

Melton T

Stratis J P

Atagün M

Gladding C

Hartman P E

Roseman S

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21 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.128.3.785-793.1976

Published

1976-12-00

Pages

785-93

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC232769

Subset

External Links

PubMed source DOI: 10.1128/jb.128.3.785-793.1976 PMC Full Text View journal details

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Fosfomycin resistance: selection method for internal and extended deletions of the phosphoenolpyruvate:sugar phosphotransferase genes of Salmonella typhimurium.

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