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PMID: 791932 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

3-Deoxy-3-fluoro-D-glucose-resistant Salmonella typhimurium mutants defective in the phosphoenolpyruvate:glycose phosphotransferase system.

Journal of bacteriology ·Vol. 128 ·No. 3 ·1976-12-00 ·Pages 794-800

Melton T, Kundig W, Hartman PE, Meadow N

Abstract

Three classes of phosphotransferase system mutants in Salmonella typhimurium were selected through their resistance to 3-deoxy-3-fluoro-D-glucose (DFG). Strains with mutations in the ptsH (HPr) and/or pts I (enzyme I) genes were selected on medium containing lactate plus DFG. Strains with mutations in ptsH but not pstI were selected on medium containing fructose plus DFG. Clones isolated from fructose plus DFG semisolid plates and selected for ability to swarm were mutant in either ptsH or ptsG. Mutants of the latter class were defective in enzyme IIB', a membrane component of the glucose transport system. Some pleiotropic properties of one representative ptsG mutant are described.

MeSH Terms
Biological Transport, Active Chromosome Mapping Chromosomes, Bacterial Deoxy Sugars/pharmacology Deoxyglucose/analogs & derivatives,pharmacology Drug Resistance, Microbial Fructose/metabolism Genes Glucose/metabolism Mannose/metabolism Mutation Phosphotransferases/biosynthesis Salmonella typhimurium/drug effects,enzymology
Chemicals
Deoxy Sugars Fructose Deoxyglucose Phosphotransferases Glucose Mannose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Melton T
Kundig W
Hartman P E
Meadow N
References (15)
15 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.128.3.794-800.1976
Published
1976-12-00
Pages
794-800
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC232770
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.128.3.794-800.1976 PMC Full Text View journal details
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