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PMID: 4897910 Published · ppublish English Journal Article

Isolation of adenyl cyclase from Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 63 ·No. 1 ·1969-05-00 ·Pages 86-92

Tao M, Lipmann F

Abstract

We have found a soluble cyclase, using for assay radioactively marked ATP as precursor. The reaction product was isolated by thin-layer chromatography and identified by specific degradation. After homogenization, part of the activity remained in the particulate fraction but could be easily extracted. The cyclase was concentrated 100-fold by conventional methods. The enzyme has a Mg(++) requirement and is inhibited by fluoride and inorganic pyrophosphate.

MeSH Terms
Adenine Nucleotides Adenylyl Cyclases/isolation & purification Chromatography, Thin Layer Enzymes/isolation & purification Escherichia coli/enzymology Magnesium Methods
Chemicals
Adenine Nucleotides Enzymes Adenylyl Cyclases Magnesium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tao M
Lipmann F
References (11)
11 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.63.1.86
Published
1969-05-00
Pages
86-92
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC534038
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.63.1.86 PMC Full Text View journal details
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