Abstract
Eukaryotic cells organize their contents through trafficking along cytoskeletal filaments. The leading edge of a typical metazoan cytoskeleton consists of a dense and complex arrangement of cortical actin. A dendritic mesh is found across the broad lamellopodium, with long parallel bundles at microspikes and filopodia. It is currently unclear whether and how myosin motors identify the few actin filaments that lead to the correct destination, when presented with many similar alternatives within the cortex. Here we show that myosin X, an actin-based motor that concentrates at the distal tips of filopodia, selects the fascin-actin bundle at the filopodial core for motility. Myosin X moves individual actin filaments poorly in vitro, often supercoiling actin into plectonemes. However, single myosin X motors move robustly and processively along fascin-actin bundles. This selection requires only parallel, closely spaced filaments, as myosin X is also processive on artificial actin bundles formed by molecular crowding. Myosin X filopodial localization is perturbed in fascin-depleted HeLa cells, demonstrating that fascin bundles also direct motility in vivo. Our results demonstrate that myosin X recognizes the local structural arrangement of filaments in long bundles, providing a mechanism for sorting cargo to distant target sites.
MeSH Terms
Actin Cytoskeleton/metabolism
Animals
Carrier Proteins/genetics
Cattle
Cell Movement
HeLa Cells
Humans
Microfilament Proteins/genetics
Molecular Motor Proteins
Myosins/metabolism,physiology
Pseudopodia/metabolism
RNA, Small Interfering/pharmacology
Chemicals
Carrier Proteins
Microfilament Proteins
Molecular Motor Proteins
RNA, Small Interfering
fascin
Myosins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Nagy Stanislav
Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA.
Ricca Benjamin L
Norstrom Melanie F
Courson David S
Brawley Crista M
Smithback Philip A
Rock Ronald S
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