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PMID: 17954606 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

The motor activity of myosin-X promotes actin fiber convergence at the cell periphery to initiate filopodia formation.

The Journal of cell biology ·Vol. 179 ·No. 2 ·2007-10-22 ·Pages 229-38

Tokuo H, Mabuchi K, Ikebe M

Abstract

Filopodia are actin-rich fingerlike protrusions found at the leading edge of migrating cells and are believed to play a role in directional sensing. Previous studies have shown that myosin-X (myoX) promotes filopodia formation and that this is mediated through its ability to deliver specific cargoes to the cell periphery (Tokuo, H., and M. Ikebe. 2004. Biochem Biophys. Commun. 319:214-220; Zhang, H., J.S. Berg, Z. Li, Y. Wang, P. Lang, A.D. Sousa, A. Bhaskar, R.E. Cheney, and S. Stromblad. 2004. Nat. Cell Biol. 6:523-531; Bohil, A.B., B.W. Robertson, and R.E. Cheney. 2006. Proc. Natl. Acad. Sci. USA. 103:12411-12416; Zhu, X.J., C.Z. Wang, P.G. Dai, Y. Xie, N.N. Song, Y. Liu, Q.S. Du, L. Mei, Y.Q. Ding, and W.C. Xiong. 2007. Nat. Cell Biol. 9:184-192). In this study, we show that the motor function of myoX and not the cargo function is critical for initiating filopodia formation. Using a dimer-inducing technique, we find that myoX lacking its cargo-binding tail moves laterally at the leading edge of lamellipodia and induces filopodia in living cells. We conclude that the motor function of the two-headed form of myoX is critical for actin reorganization at the leading edge, leading to filopodia formation.

MeSH Terms
Actins/metabolism Animals COS Cells Cell Polarity Cell Survival Chlorocebus aethiops Dimerization Mice Molecular Motor Proteins/metabolism Myosins/chemistry,deficiency,metabolism NIH 3T3 Cells Pseudopodia/metabolism Recombinant Fusion Proteins/metabolism
Chemicals
Actins Molecular Motor Proteins Myo10 protein, mouse Recombinant Fusion Proteins Myosins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tokuo Hiroshi
Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA.
Mabuchi Katsuhide
Ikebe Mitsuo
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200703178
Published
2007-10-22
Pages
229-38
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2064759
Subset
IM
Grants
NIAMS NIH HHS · AR048898 · United States
NIDCD NIH HHS · R01 DC006103 · United States
NIAMS NIH HHS · AR048526 · United States
NIAMS NIH HHS · R01 AR048526 · United States
NIDCD NIH HHS · DC006103 · United States
NIAMS NIH HHS · R01 AR048898 · United States
External Links
PubMed source DOI: 10.1083/jcb.200703178 PMC Full Text View journal details
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