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PMID: 11707568 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Myosin VI is a processive motor with a large step size.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 24 ·2001-11-20 ·Pages 13655-9

Rock RS, Rice SE, Wells AL, Purcell TJ, Spudich JA, Sweeney HL

Abstract

Myosin VI is a molecular motor involved in intracellular vesicle and organelle transport. To carry out its cellular functions myosin VI moves toward the pointed end of actin, backward in relation to all other characterized myosins. Myosin V, a motor that moves toward the barbed end of actin, is processive, undergoing multiple catalytic cycles and mechanical advances before it releases from actin. Here we show that myosin VI is also processive by using single molecule motility and optical trapping experiments. Remarkably, myosin VI takes much larger steps than expected, based on a simple lever-arm mechanism, for a myosin with only one light chain in the lever-arm domain. Unlike other characterized myosins, myosin VI stepping is highly irregular with a broad distribution of step sizes.

MeSH Terms
Actin Cytoskeleton/metabolism Actins/metabolism Animals Carrier Proteins/genetics,metabolism Chickens Green Fluorescent Proteins Luminescent Proteins/genetics,metabolism Microfilament Proteins/metabolism Molecular Motor Proteins/metabolism Myosin Heavy Chains/genetics,metabolism Recombinant Fusion Proteins/genetics,metabolism Swine
Chemicals
Actins Carrier Proteins Luminescent Proteins Microfilament Proteins Molecular Motor Proteins Recombinant Fusion Proteins myosin VI fascin Green Fluorescent Proteins Myosin Heavy Chains
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rock R S
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA.
Rice S E
Wells A L
Purcell T J
Spudich J A
Sweeney H L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.191512398
Published
2001-11-20
Epub
2001-00-13
Pages
13655-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC61096
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.191512398 PMC Full Text View journal details
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