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PMID: 18522725 Published · epublish English Journal Article

Compatible solute influence on nucleic acids: many questions but few answers.

Saline systems ·Vol. 4 ·2008-06-03 ·Pages 6

Kurz M

Abstract

Compatible solutes are small organic osmolytes including but not limited to sugars, polyols, amino acids, and their derivatives. They are compatible with cell metabolism even at molar concentrations. A variety of organisms synthesize or take up compatible solutes for adaptation to extreme environments. In addition to their protective action on whole cells, compatible solutes display significant effects on biomolecules in vitro. These include stabilization of native protein and nucleic acid structures. They are used as additives in polymerase chain reactions to increase product yield and specificity, but also in other nucleic acid and protein applications. Interactions of compatible solutes with nucleic acids and protein-nucleic acid complexes are much less understood than the corresponding interactions of compatible solutes with proteins. Although we may begin to understand solute/nucleic acid interactions there are only few answers to the many questions we have. I summarize here the current state of knowledge and discuss possible molecular mechanisms and thermodynamics.

Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kurz Matthias
Institut für Mikrobiologie & Biotechnologie, Rheinische Friedrich Wilhelms-Universität Bonn, Bonn, Germany. mkurz@uni-bonn.de
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Article Info
Journal
Saline systems
Abbr.
Saline Syst
ISSN
1746-1448
DOI
10.1186/1746-1448-4-6
Email
mkurz@uni-bonn.de
Published
2008-06-03
Epub
2008-00-03
Pages
6
Language
English
Region
England
NLM ID
101245795
PMCID
PMC2430576
External Links
PubMed source DOI: 10.1186/1746-1448-4-6 PMC Full Text
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