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PMID: 15788751 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Polyamines preferentially interact with bent adenine tracts in double-stranded DNA.

Nucleic acids research ·Vol. 33 ·No. 6 ·2005-00-00 ·Pages 1790-803

Lindemose S, Nielsen PE, Møllegaard NE

Abstract

Polyamines, such as putrescine, spermidine and spermine, have indirectly been linked with the regulation of gene expression, and their concentrations are typically increased in cancer cells. Although effects on transcription factor binding to cognate DNA targets have been demonstrated, the mechanisms of the biological action of polyamines is poorly understood. Employing uranyl photo-probing we now demonstrate that polyamines at submillimolar concentrations bind preferentially to bent adenine tracts in double-stranded DNA. These results provide the first clear evidence for the sequence-specific binding of polyamines to DNA, and thereby suggest a mechanism by which the cellular effects of polyamines in terms of differential gene transcriptional activity could, at least partly, be a direct consequence of sequence-specific interactions of polyamines with promoters at the DNA sequence level.

MeSH Terms
Adenine/metabolism Binding Sites DNA/chemistry,metabolism,radiation effects Humans Nucleic Acid Conformation Polyamines/metabolism,pharmacology Promoter Regions, Genetic Uranyl Nitrate/chemistry
Chemicals
Polyamines Uranyl Nitrate DNA Adenine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lindemose Søren
Department of Medical Biochemistry and Genetics, The Panum Institute, University of Copenhagen Blegdamsvej 3C, 2200 Copenhagen N, Denmark.
Nielsen Peter E
Møllegaard Niels Erik
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gki319
Published
2005-00-00
Epub
2005-00-23
Pages
1790-803
Language
English
Region
England
NLM ID
0411011
PMCID
PMC1069516
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gki319 PMC Full Text View journal details
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