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PMID: 1851992 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functional domains of the poliovirus receptor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 10 ·1991-05-15 ·Pages 4104-8

Koike S, Ise I, Nomoto A

Abstract

A number of mutant cDNAs of the human poliovirus receptor were constructed to identify essential regions of the molecule as the receptor. All mutant cDNAs carrying the sequence coding for the entire N-terminal immunoglobulin-like domain (domain I) confer permissiveness for poliovirus to mouse L cells, but a mutant cDNA lacking the sequence for domain I does not. The transformants permissive for poliovirus were able to bind the virus and were also recognized by monoclonal antibody D171, which competes with poliovirus for the cellular receptor. These results strongly suggest that the poliovirus binding site resides in domain I of the receptor. Mutant cDNAs for the sequence encoding the intracellular peptide were also constructed and expressed in mouse L cells. Susceptibility of these cells to poliovirus revealed that the entire putative cytoplasmic domain is not essential for virus infection. Thus, the cytoplasmic domain of the molecule appears not to play a role in the penetration of poliovirus.

MeSH Terms
Animals Base Sequence Chromosome Deletion Cloning, Molecular DNA/genetics Gene Expression L Cells/microbiology Mice Molecular Sequence Data Mutagenesis Nucleic Acid Hybridization Poliovirus/physiology Receptors, Virus/genetics,physiology Structure-Activity Relationship Transfection
Chemicals
Receptors, Virus DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Koike S
Department of Microbiology, Tokyo Metropolitan Institute of Medical Science, Japan.
Ise I
Nomoto A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.10.4104
Published
1991-05-15
Pages
4104-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51606
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.10.4104 PMC Full Text View journal details
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