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PMID: 2168959 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Neutralization of poliovirus by cell receptors expressed in insect cells.

Journal of virology ·Vol. 64 ·No. 10 ·1990-10-00 ·Pages 4697-702

Kaplan G, Freistadt MS, Racaniello VR

Abstract

To examine the interaction of the poliovirus receptor (PVR) with virus and the role of the PVR in virus entry, the PVR was expressed in insect cells. Poliovirus bound to insect cells infected with a recombinant baculovirus (AcPVR) carrying cDNA encoding the PVR. Antibodies raised against PVR expressed in bacteria immunoprecipitated a 67-kilodalton polypeptide from cytoplasmic extracts of AcPVR-infected cells. Treatment of AcPVR-infected cells with tunicamycin revealed that the PVR is a glycoprotein containing N-glycosidic linkages and that carbohydrate accounts for nearly 50% of its molecular weight as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. When PVR was solubilized from AcPVR-infected insect cells and incubated with poliovirus, viral infectivity was neutralized. Sedimentation analysis revealed that irreversibly altered 135S particles were formed after incubation of poliovirus at 37 degrees C with solubilized extracts of AcPVR-infected insect cells. These results demonstrate that poliovirus eclipse may result from interaction with the cell receptor at neutral pH in the absence of membranes and suggest that soluble receptors may be effective antiviral agents against picornaviruses.

MeSH Terms
Animals Cell Line DNA, Viral/genetics,isolation & purification Enzyme-Linked Immunosorbent Assay HeLa Cells/metabolism Humans Insect Viruses/genetics Insecta Neutralization Tests Poliovirus/physiology Receptors, Virus/analysis,genetics,physiology Recombinant Proteins/analysis,metabolism Virion/physiology
Chemicals
DNA, Viral Receptors, Virus Recombinant Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kaplan G
Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Freistadt M S
Racaniello V R
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.10.4697-4702.1990
Published
1990-10-00
Pages
4697-702
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247955
Subset
IM
Grants
NIAID NIH HHS · AI20017 · United States
External Links
PubMed source DOI: 10.1128/JVI.64.10.4697-4702.1990 PMC Full Text View journal details
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