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PMID: 1848015 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Drosophila stimulatory G protein alpha subunit activates mammalian adenylyl cyclase but interacts poorly with mammalian receptors: implications for receptor-G protein interaction.

Quan F, Thomas L, Forte M

Abstract

Heterotrimeric guanine nucleotide binding proteins (G proteins) transduce signals from cell-surface receptors to intracellular effector proteins. Two forms of stimulatory G protein (Gs) alpha-like subunit have been described in Drosophila melanogaster. To examine the function of these subunits we have used vaccinia virus vectors to express both proteins in cyc- cells, a murine S49 cell line deficient for Gs alpha activity. Receptor-independent activation of each Drosophila Gs alpha has demonstrated that both forms are capable of activating mammalian adenylyl cyclase and thus have the activity expected of stimulatory G proteins. However, the Drosophila Gs alpha subunits interact poorly with mammalian Gs-coupled receptors. These observations have helped to identify a region of high variability in Gs alpha proteins that may be important for receptor interactions.

MeSH Terms
Adenylyl Cyclases/metabolism Amino Acid Sequence Animals Antisense Elements (Genetics) Base Sequence Cell Line Cholera Toxin/pharmacology Cloning, Molecular Drosophila/metabolism Enzyme Activation GTP-Binding Proteins/genetics,metabolism Genetic Vectors Kinetics Macromolecular Substances Molecular Sequence Data Mutagenesis, Site-Directed Receptors, Cell Surface/physiology Recombinant Proteins/metabolism Restriction Mapping Sequence Homology, Nucleic Acid Signal Transduction Vaccinia virus/genetics
Chemicals
Antisense Elements (Genetics) Macromolecular Substances Receptors, Cell Surface Recombinant Proteins Cholera Toxin GTP-Binding Proteins Adenylyl Cyclases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Quan F
Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97201.
Thomas L
Forte M
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42 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1991-03-01
Pages
1898-902
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51133
Subset
IM
Grants
NIDDK NIH HHS · DK37274 · United States
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